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pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A-H548A-R551A

Plasmid

#238317

Purpose

ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC TtoS

Plasmid

#226608

Purpose

All threonines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC QtoA

Plasmid

#226609

Purpose

All glutamines to alanine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

6*His-TEV-FUS LC QtoA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->G

Plasmid

#226597

Purpose

12 serines to glycines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->A (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->A

Plasmid

#226598

Purpose

12 serines to alanines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->A (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->N

Plasmid

#226599

Purpose

12 serines to asparagines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->N (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to asparagine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#1

Plasmid

#226603

Purpose

4 serines to alanine at residue positions 30, 44, 48, 53

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC S->A#1 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#2

Plasmid

#226604

Purpose

4 serines to alanine at residue positions 70, 84, 89, 95

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#2 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#3

Plasmid

#226605

Purpose

4 serines to alanine at residue positions 108, 117, 119, 127

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#3 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC StoG

Plasmid

#226607

Purpose

All serines to glycines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC TtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All threonine to serine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A-K556A-K644A

Plasmid

#238328

Purpose

ScVip1-536-1107-K554A-K556A-K644A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A-K556A-K644A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-P553V-G646V-G647A

Plasmid

#238322

Purpose

ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-P553V-G646V-G647A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K817S-D819A-S821A

Plasmid

#238326

Purpose

ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K817S-D819A-S821A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TCR001H

Plasmid

#233449

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR001H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR001H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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TCR004H

Plasmid

#233450

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR004H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR004H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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TCR005H

Plasmid

#233451

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR005H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR005H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TCR008H

Plasmid

#233452

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR008H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR008H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TCR010H

Plasmid

#233453

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR010H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR010H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
TCR013H

Plasmid

#233455

Purpose

Expresses ncHLAp-reactive T cell receptor (TCR013H)

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Insert

TCR013H

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgCBS-1

Plasmid

#230081

Purpose

Crispr knock out human CBS gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgCBS-2

Plasmid

#230082

Purpose

Crispr knock out human CBS gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

CBS (CBS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR v2-sgL2HGDH-1

Plasmid

#230083

Purpose

Crispr knock out human L2HGDH gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

L2HGDH (L2HGDH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR v2-sgL2HGDH-2

Plasmid

#230084

Purpose

Crispr knock out human L2HGDH gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

L2HGDH (L2HGDH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR v2-sgL2HGDH-3

Plasmid

#230085

Purpose

Crispr knock out human L2HGDH gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

L2HGDH (L2HGDH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-MUT-P2A-GFP

Plasmid

#229406

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-TC1-2_DM

Plasmid

#217081

Purpose

Donor plasmid of TC1-2_DM Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of TC1-2_DM transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMV-PROTOP

Plasmid

#217091

Purpose

Donor plasmid of PROTOP Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of PROTOP transposon

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV-Tc1-8_Xt

Plasmid

#217092

Purpose

Donor plasmid of Tc1-8_Xt Transposon

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tc1-8_Xt transposon

Expression

Mammalian

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge