We narrowed to 14,584 results for: pET

1. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

   Plasmid

   #178035

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted and Changed T(8…

   Promoter

   T7LacI

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-PLS-KP2-E67A_rpoA-U

   Plasmid

   #190959

   Purpose

   Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:E67A

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-PLS-KP6-A66S_rpoA-C

   Plasmid

   #190968

   Purpose

   Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:A66S

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-PLS-KP2-A66S_rpoA-U

   Plasmid

   #190957

   Purpose

   Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:A66S

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-PLS-KP3-A66S_rpoA-U

   Plasmid

   #190961

   Purpose

   Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:A66S

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-PLS-KP3-E67A_rpoA-U

   Plasmid

   #190963

   Purpose

   Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:E67A

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-PLS-KP6-E67A_rpoA-C

   Plasmid

   #190972

   Purpose

   Test the C-to-U editing activity of KP6:E67A on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   DYW-KP:E67A

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Arabidopsis thaliana Atm3 pET21a+

   Plasmid

   #172321

   Purpose

   Expresses AtAtm3 in E.coli

   Depositor

   Douglas Rees

   Article

   Fan et al Elife. 2022 Mar 25;11. pii: 76140. doi:

   Insert

   Arabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTln1-F2F3-P229L-pET151

   Plasmid

   #177879

   Purpose

   Expresses mouse Talin 1 F2F3-P229L domains in bacterial cells

   Depositor

   Ben Goult

   Article

   Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

   Insert

   mouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   changed Proline 229 to Leucine (P229L)

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mTln1-F2F3-P229S-pET151

    Plasmid

    #177878

    Purpose

    Expresses mouse Talin 1 F2F3-P229S domains in bacterial cells

    Depositor

    Ben Goult

    Article

    Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

    Insert

    mouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    changed Proline 229 to Serine (P229S)

    Promoter

    T7

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET28a-cpLumiLuc-amylase sensor

    Plasmid

    #186293

    Purpose

    Expression alpha-amylase sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-amylase sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET28a-cpLumiLuc-HSA sensor

    Plasmid

    #186294

    Purpose

    Expression Human serum albumin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-HSA sensor
    

    Use

    Tags

    HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET303-ColE1-TR-E192C-del1-40

    Plasmid

    #180235

    Purpose

    Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    Colicin E1
    

    Use

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Truncation of ColE1 containing T and R domains (r…

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-15b-LanCL1-H219A

    Plasmid

    #154190

    Purpose

    To express LanCL1-H219A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    His219->Ala219

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-15b-LanCL1-C322A

    Plasmid

    #154191

    Purpose

    To express LanCL1-C322A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL1
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys322->Ala322

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15 PemrR-gfp biosensor

    Plasmid

    #185793

    Purpose

    The pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.

    Depositor

    Steven Hallam

    Article

    Ho et al ACS Synth Biol. 2018 Feb 16;7(2):392-398

    Insert

    pEmrR-gfp
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PemrR

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b MOSPD2 RD/LD [282-490]

    Plasmid

    #186470

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b MOSPD2 [282-490]

    Plasmid

    #186469

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [282-490]

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET30-T7-lacO-His-sfGFP

    Plasmid

    #180754

    Purpose

    pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

    Depositor

    Michael Black

    Article

    Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

    Insert

    superfolder green fluorescent protein
    

    Use

    Tags

    strep tag

    Expression

    Bacterial

    Mutation

    G140C- Please see depositor comments

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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