We narrowed to 3,807 results for: golden gate

1. pLentiX1-[TRE3G-TS.FF6x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235321

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating mRuby2, design 2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiX1-[TRE3G-mRuby2-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235313

   Purpose

   Lentiviral expression of mRuby2 with 3' microRNA target site only (open-loop)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235320

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235319

   Purpose

   Lentiviral expression of ComMAND open-loop circuit regulating mRuby2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235314

   Purpose

   Lentiviral expression of mRuby2 with 3' microRNA target site only (closed-loop)

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiX1-[TRE3G-TS.FF4x1-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235322

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 2

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLentiX1-[TRE3G-TS.FF4x1-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

   Plasmid

   #235324

   Purpose

   Lentiviral expression of ComMAND closed-loop circuit regulating mRuby2, design 3

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mRuby2
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G (3rd-generation Tet system)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-AtmiR173aTS-B/c

   Plasmid

   #227964

   Purpose

   Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Inserts

   B/c
   AtmiR173aTS
   

   Promoter

   None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMDC32B-AtmiR173aTS-B/c

   Plasmid

   #227965

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Inserts

   B/c
   AtmiR173aTS
   

   Expression

   Plant

   Promoter

   35S and None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-NbmiR482aTS-B/c

    Plasmid

    #227966

    Purpose

    Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Inserts

    B/c
    NbmiR482aTS
    

    Promoter

    None

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

    Plasmid

    #234371

    Purpose

    T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Promoter

    Arabidopsis Ubi10 and MTAP1 (synthetic)

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.minBG-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231362

    Purpose

    minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231363

    Purpose

    Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. B8-pVTCB052

    Plasmid

    #231302

    Purpose

    Plasmid used for expression of AI-designed GFP protein B8 in E. coli

    Depositor

    Alexander Rives

    Insert

    B8
    

    Expression

    Bacterial

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p35S-FHA-AtAGO4_gDNA

    Plasmid

    #216838

    Purpose

    N-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under regulation of CaMV 35S promoter

    Depositor

    Arturo Marí-Ordóñez

    Article

    Dombey et al bioRxiv 2024.04.03.587901; doi: https://

    Insert

    ARGONAUTE4 (AGO4 Mustard Weed)
    

    Tags

    Flag-HA

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pM-mKate2

    Plasmid

    #228629

    Purpose

    Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mKate2
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pM-LSSmOrange

    Plasmid

    #228619

    Purpose

    Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    LSSmOrange
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pM-mBeRFP

    Plasmid

    #228620

    Purpose

    Mammalian expression of mBeRFP and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mBeRFP
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM-mTFP1

    Plasmid

    #228621

    Purpose

    Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mTFP1
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pM-CyOFP1

    Plasmid

    #228623

    Purpose

    Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    CyOFP1
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge