We narrowed to 8,749 results for: Dos

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myc-BioID-Rab7

Plasmid

#121049

Purpose

Expresses myc tagged BioID and human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
Rab7A

Tags

myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARα

Plasmid

#119913

Purpose

Mammalian expression of the tAKARα sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARα

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5060

Plasmid

#128716

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

Insert

shcN-hopN1

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-VAMP2:HRP

Plasmid

#60659

Purpose

Labels synaptic vesicles for electron microscopy

Depositor

Scott Sternson

Article

Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

Insert

VAMP2:HRP

Use

AAV and Cre/Lox

Tags

Myc

Promoter

CAG

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pLenti6.3-SaCas9-BlastR

Plasmid

#220966

Purpose

Expresses human codon-optimized SaCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SaCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_VDR_Hormone-recep

Plasmid

#109962

Purpose

Protein expression and purification of VDR_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VDR_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJH1371

Plasmid

#199631

Purpose

Prgef-1 GPI YFP unc-54 3' UTR C.elegans Pan-neural expression of GPI-YFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GPI

Tags

YFP

Expression

Worm

Promoter

Pregf-1

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Ki67 (LR)-mNeonGreen-IRESpuro2

Plasmid

#183743

Purpose

Expresses Ki67 (LR)-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 LR domain (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

LR domain of Ki67

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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RnBarr2-mVenus

Plasmid

#137793

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNAJB1_DNAJ

Plasmid

#109918

Purpose

Protein expression and purification of DNAJB1_DNAJ

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNAJB1_DNAJ

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB120

Plasmid

#68574

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Zfp536

Plasmid

#45845

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Zfp536 (Zfp536 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-KRaION2

Plasmid

#177800

Purpose

Bacterial expression of green fluorescent potassium indicator KRaION2

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

KRaION2

Expression

Bacterial

Promoter

araB

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTpal-fdc

Plasmid

#78288

Purpose

Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

Depositor

David Nielsen

Article

McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

Inserts

PAL2 (PAL2 Mustard Weed)
FDC1 (FDC1 Budding Yeast)

Expression

Bacterial

Mutation

*see below

Promoter

trc

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPF45

Plasmid

#110044

Purpose

Protein expression and purification of SPF45

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPF45 (RBM17 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Actin binding domain of Ezrin

Plasmid

#62381

Purpose

Expresses the actin binding domain of ezrin. Amino acids 552-586.

Depositor

Guillaume Charras

Article

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Actin binding domain of ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

contains aa's 552-586

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

Plasmid

#173874

Purpose

Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSMP-RING1_2

Plasmid

#36356

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

RING1 (RING1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-L3-SpCas9-HF1-2A-EGFP-L2

Plasmid

#141048

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF1-2A-EGFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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