We narrowed to 159,258 results for: addgene

1. LC3B sgRNA

   Plasmid

   #207556

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CACTGAATACCAGCGCCTAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LacZ_pHIVEGFP

   Plasmid

   #191251

   Purpose

   Expresses LacZ in mammalian cells (used as a control)

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

   Insert

   LacZ
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p8266 LentiCRISPR v2 hygro sgNT-1

   Plasmid

   #193977

   Purpose

   Expression of spCas9 and non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p8267 LentiCRISPR v2 hygro sgNT-2

   Plasmid

   #193978

   Purpose

   Expression of spCas9 and non-targeting control sgRNA

   Depositor

   Elizabeth White

   Article

   Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LentiGuide-TagRFP-2A-BSD

   Plasmid

   #167930

   Purpose

   Lentiviral gRNA expression with TagRFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ptet-RFP-shR-rtTA

   Plasmid

   #35625

   Purpose

   Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

   Depositor

   Howard Gu

   Article

   Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

   Type

   Empty backbone

   Use

   AAV and RNAi; Tet inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ptet

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LES2A-CRE

   Plasmid

   #167933

   Purpose

   Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   mScarlet-P2A-CRE
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGH224_sgRNA_2xMS2_Puro

   Plasmid

   #85413

   Purpose

   hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hIRE1a wt

   Plasmid

   #20744

   Depositor

   Fumihiko Urano

   Article

   Lipson et al PLoS ONE. 2008 . 3(2):e1648.

   Insert

   IRE1 (ERN1 Human)
   

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVCT-tTR-KRAB

    Plasmid

    #11643

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    CAG promoters, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge