We narrowed to 3,807 results for: golden gate

1. pSC5GGv1

   Plasmid

   #194645

   Purpose

   pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCKC775

   Plasmid

   #226626

   Purpose

   piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
   EF1a promoter, sfGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   7xTCF-LEF and EF1a

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCKC764

   Plasmid

   #226625

   Purpose

   piggyBAC pCAG-hCas9-T2A-Unbiased3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
   EF1a promoter, blasticidin resistance
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV and EF1a

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCKC776

   Plasmid

   #226627

   Purpose

   piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
   EF1a promoter, mCherry
   

   Use

   Synthetic Biology

   Tags

   oxygen dependent degron (ODD)

   Expression

   Mammalian

   Promoter

   4xHRE_YB TATA and EF1a

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB123 - pL0_rLUC-I (CDS1)

   Plasmid

   #123181

   Purpose

   Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants

   Depositor

   Carolyn Lee-Parsons, Erin Cram

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
   

   Use

   Luciferase; Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites; codon-optimised for plants

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MT-AvrXa10-FT

   Plasmid

   #234373

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

   Depositor

   Michael Smanski

   Article

   Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

   Inserts

   NbGP41-GFP-AvrXa10-GB1
   dCas9-24XGP41
   gRNAs targeting FT locus
   RFP
   

   Use

   Synthetic Biology

   Tags

   GB1, GFP, and GP41

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL11060

   Plasmid

   #68264

   Purpose

   Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-ITR-EcYtR-mCherry

   Plasmid

   #217362

   Purpose

   Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ133-RZ-Cas12j2

   Plasmid

   #189784

   Purpose

   Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC5GGv2

    Plasmid

    #188604

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 2)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF004-sgRNA-shuffle-in

    Plasmid

    #104440

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    U6 promoter

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOP22-5'UTR-BBa_B0034

    Plasmid

    #216626

    Purpose

    NanoLuc reporter plasmid to optimize chloroplast cell-free systems with RBS BBa_B0034 in 5'UTR

    Depositor

    Henrike Niederholtmeyer

    Article

    Bohm et al ACS Synth Biol. 2024 Aug 16;13(8):2412-2

    Insert

    T7p-5'B0034-NanoLuc-3'TMV
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid

    #123180

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    

    Use

    Luciferase; Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

    Plasmid

    #198054

    Purpose

    All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

    Depositor

    Lacra Bintu

    Article

    Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

    Inserts

    rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
    3xFLAG-insert
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    TRE3G and pEF

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB65 - pL0_tGFP-I (CDS1)

    Plasmid

    #123182

    Purpose

    Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
    

    Use

    Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites; codon-optimised for plants

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNL2119 pARF7 GG0-GG1

    Plasmid

    #195910

    Purpose

    Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pARF7 (NPH4 Mustard Weed)
    

    Use

    Synthetic Biology

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-OiRS3GG

    Plasmid

    #98589

    Purpose

    Expresses default target RNA (gI intron) and pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

    Depositor

    Lydia Contreras

    Article

    Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

    Inserts

    group I intron
    PheS cassette to be selectively replaced by unique asRNA probes
    

    Expression

    Bacterial

    Promoter

    pBAD and pLtetO

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTW411b

    Plasmid

    #246583

    Purpose

    different combination of A. thaliana and N. tabacum Rubisco assembly factors

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    NtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 )
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), VEGFR1 CTEVp chain with ZF6a in MGEV

    Plasmid

    #244188

    Purpose

    Multigene vector for constitutive expression of mNeonGreen, VEGFR2 NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    mNeonGreen; VEGFR2 NTEVp chain with NTEVp 75S mutant; VEGFR1 CTEVp chain with ZF6 synTF
    

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NTEVp_75S, CTEVp_190K

    Promoter

    hEF1a, CMV

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV

    Plasmid

    #244190

    Purpose

    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a

    Depositor

    Joshua Leonard

    Article

    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

    Insert

    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF
    

    Use

    Synthetic Biology

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    NTEVp_75S, CTEVp_190K

    Promoter

    hEF1a, CMV

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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