We narrowed to 6,905 results for: crispr cas9 plasmids

1. pDonor_sU6

   Plasmid

   #69351

   Purpose

   sgRNA scaffold and synthetic U6 promoter

   Depositor

   Andrea Ventura

   Article

   Vidigal et al Nat Commun. 2015 Aug 17;6:8083. doi: 10.

   Insert

   sU6 promoter and sgRNA scaffold flanked by BbsI sites
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   synthetic U6 promoter

   Available since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. U6>sgRNA(F+E)

   Plasmid

   #59986

   Purpose

   Empty vector for sgRNA cloning (F+E modified backbone)

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ciinte.U6

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAH233

   Plasmid

   #121144

   Purpose

   Expression of gRNA with BbsI placeholder for target cloning

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-CASANOVA

   Plasmid

   #113036

   Purpose

   AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTJK459

   Plasmid

   #138008

   Purpose

   Empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Tami Kingsbury

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   U-A flip and hairpin extension: cgtttAagagctaTGCT…

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

   Plasmid

   #117168

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMpGE_En03

   Plasmid

   #71535

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Sumihare Noji, Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC gRNA Shuttle

   Plasmid

   #47024

   Purpose

   Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Insert

   gRNA Shuttle
   

   Use

   CRISPR; Cas9

   Tags

   Expression

   Plant

   Mutation

   G to T cloning mutation at position 323

   Promoter

   Medicago truncatula U6.6

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBlu/gRNA

    Plasmid

    #59188

    Purpose

    Serves as a shuttle vector for target oligo before insertion into Cas9 destination vector

    Depositor

    Robert Stupar

    Article

    Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

    Insert

    Guide RNA Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6 (Arabidopis)

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge