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We narrowed to 8,068 results for: gnal

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV and cmv

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pPGK-T7/2-CD44v6 (-cyt)

Plasmid

#137817

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region, R296K (please see dep…

Promoter

PGK

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2_antisense-control

Plasmid

#42174

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pRepair-SGFP2-CTNNB1

Plasmid

#153430

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SGFP2

Expression

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-mCherry-p115(S240A)

Plasmid

#181997

Purpose

Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.

Depositor

Jin Zhang

Article

Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

Insert

mCherry-p115(S240A) (ARHGEF1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Serine 240 changed to alanine in p115.

Promoter

CMV

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-CA576-Z

Plasmid

#89108

Purpose

mammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576

Depositor

Elizabeth Wilson

Article

Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

Insert

hAR-C576A (AR Human)

Use

Tags

Expression

Mammalian

Mutation

C576A

Promoter

CMV

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only
pRK5-LYCHOS (GPR155) WT-HA

Plasmid

#199660

Purpose

Transient expression of LYCHOS (GPR155) WT

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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PRKCDPB_pLX307

Plasmid

#98359

Purpose

Lentiviral expression of PRKCDPB

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

PRKCDPB (CAVIN3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R8P

Promoter

E1Fa

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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PF3D7_1136200-bio

Plasmid

#47730

Purpose

Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised PF3D7_1136200

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin replaced…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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GLI1_pLENTI-CAG-IRES-GFP

Plasmid

#176992

Purpose

Mammalian lentiviral expression vector encoding GLI1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-CTNNB1-ATG

Plasmid

#153429

Purpose

Expresses a gRNA that overlaps the startcodon of human CTNNB1

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 gRNA (CTNNB1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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GLI1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176968

Purpose

Mammalian expression vector encoding GLI1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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RAB10_pLX307

Plasmid

#98365

Purpose

Lentiviral expression of RAB10

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA6-C-3XFLAG-h-Lmbr1l

Plasmid

#123462

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Limb development membrane protein 1 like (LMBR1L Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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RNF138_pLX307

Plasmid

#98368

Purpose

Lentiviral expression of RNF138

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

RNF138 (RNF138 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter MUT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107010

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-HTR6-SNAPf-TRIP

Plasmid

#189617

Purpose

Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6-TRIP_alpha

Use

AAV

Tags

alphaTag in the N-term of HTR6, SNAP-tag in the C…

Expression

Mutation

Promoter

TRE

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Axin 1 (Axin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL

Plasmid

#119952

Purpose

Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

gpps
limS

Use

Tags

Expression

Bacterial

Mutation

N-terminal truncation of 56 amino acid residues (…

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-MDMX (1-120)

Plasmid

#62065

Purpose

Expression of human MDM2 (1-120) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

MDM4 (MDM4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

contains residues 1-120

Promoter

tac

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Use

Tags

TwinStrep-tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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COPE_pLX307

Plasmid

#98327

Purpose

Lentiviral expression of COPE

Depositor

Sefi Rosenbluh, William Hahn

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

COPE (COPE Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR2

Plasmid

#167001

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 2 (AR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR1

Plasmid

#167000

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 1 (AR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRepair-SYFP2-CTNNB1

Plasmid

#153432

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

Depositor

Renee van Amerongen

Article

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

SYFP2

Expression

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-INPP5ED477N

Plasmid

#202738

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

TagBFP2-FKBP-INPP5ED477N (INPP5E, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-Y766F

Plasmid

#59777

Purpose

Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-Y766F (FGFR1 Mustard Weed, Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-N-HA-Gp78

Plasmid

#123591

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

autocrine motility factor receptor (Amfr Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU1

Plasmid

#133978

Purpose

mammalian expression vector of Flag tagged RNF168 where the first motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU1

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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