We narrowed to 9,127 results for: Pol

1. pET21a - TR

   Plasmid

   #89621

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Thioredoxin
   

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-RRN3/TIF1A

   Plasmid

   #17661

   Depositor

   Tom Misteli

   Article

   Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

   Insert

   RRN3/TIF1A (RRN3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiV_Blast

   Plasmid

   #111887

   Purpose

   Lentiviral expression of cDNA with blasticidin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pgRNA

   Plasmid

   #215560

   Purpose

   Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   gRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. hMTP-flag

   Plasmid

   #138335

   Purpose

   Expresses MTP

   Depositor

   Mahmood Hussain

   Article

   Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

   Insert

   MTTP (MTTP Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. JME4599

   Plasmid

   #129660

   Purpose

   NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-ConVERGD-ConFoff-eGFP-W3SL

   Plasmid

   #218751

   Purpose

   Provides NOT intersectional (Cre NOT Flp) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ConVERGD-eGFP-WPRE

   Plasmid

   #218743

   Purpose

   Provides AND intersectional (Cre+Flp) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiV_Puro

   Plasmid

   #111886

   Purpose

   Lentiviral expression of cDNA with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-KO

    Plasmid

    #20331

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Klf4-F2A-Oct4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. 5HT2A-APEX2

    Plasmid

    #215558

    Purpose

    Stable, inducible expression of 5HT2A

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
    

    Use

    Lentiviral

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMpGWB402-er-mCherry

    Plasmid

    #186288

    Purpose

    Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

    Insert

    er-mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. IVT-MCP-Phi29

    Plasmid

    #207456

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    MCP-Phi29
    

    Expression

    Bacterial

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAP2-GCaMP3

    Plasmid

    #43917

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Insert

    GCaMP3
    

    Use

    Lentiviral

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    E247V in GCaMP3

    Promoter

    Human MAP2 promoter

    Available Since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyLigase

    Plasmid

    #51722

    Purpose

    Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

    Depositor

    Mark Howarth

    Article

    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

    Insert

    SpyLigase
    

    Tags

    His6 Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmNFL

    Plasmid

    #83127

    Purpose

    Mouse neurofilament protein L cDNA mammalian expression construct

    Depositor

    Anthony Brown

    Article

    Yan et al Cell Motil Cytoskeleton. 2007 Apr;64(4):

    Insert

    mouse NFL (Nefl Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMV306G13+FFluc

    Plasmid

    #26157

    Depositor

    Brian Robertson, Siouxsie Wiles

    Article

    Andreu et al PLoS One. 2010 May 24;5(5):e10777.

    Insert

    G13 promoter + Firefly luciferase
    

    Use

    Mycobacteria integrating vector

    Mutation

    Codon optimized for Mycobacterium tuberculosis. …

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-eGFP-MinD

    Plasmid

    #133622

    Purpose

    Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

    Depositor

    Petra Schwille

    Article

    Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

    Insert

    EGFP-MinD (minD E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEasy Flox

    Plasmid

    #11725

    Depositor

    Klaus Rajewsky

    Article

    Schenten et al Eur J Immunol. 2002 Nov . 32(11):3152-60

    Insert

    pEasyFlox
    

    Use

    Cre/Lox

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. AviTag-SpyCatcher

    Plasmid

    #72326

    Purpose

    Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    AviTag-SpyCatcher
    

    Tags

    N-term His6 tag and TEV cleavage site

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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