We narrowed to 3,807 results for: golden gate

1. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

   Plasmid

   #234372

   Purpose

   T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-24XGP41
   gRNA 1-1 and gRNA 1-2
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Promoter

   Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. DG SaCas9 GFP V3

   Plasmid

   #226961

   Purpose

   CBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. DG SaCas9 puro V3

   Plasmid

   #226960

   Purpose

   CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28:LanII-2C

   Plasmid

   #225070

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2C)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28:LanII-2A

   Plasmid

   #225068

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28:LanII-2B

   Plasmid

   #225069

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-2B)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28:LanII-57

   Plasmid

   #225074

   Purpose

   Expresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA(A) (LanII-57)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28:LanIII-6

   Plasmid

   #225076

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA(A) (LanIII-6)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28:LanII-23

   Plasmid

   #225067

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII-23)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCM0-109

    Plasmid

    #218200

    Purpose

    SV40 NLS for protein targeting to nucleus

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    SV40 NLS
    

    Use

    Synthetic Biology

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMBS-858

    Plasmid

    #218008

    Purpose

    mVenus fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBS-853

    Plasmid

    #218011

    Purpose

    mCerulean fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMBS-854

    Plasmid

    #218012

    Purpose

    mVenus fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMBS-855

    Plasmid

    #218013

    Purpose

    mCherry fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMBS-856

    Plasmid

    #218014

    Purpose

    sfGFP fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMBS-872

    Plasmid

    #218015

    Purpose

    NanoLuc luminescence reporter

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    NanoLuc
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMBS-860

    Plasmid

    #218016

    Purpose

    mVenus fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMBS-861

    Plasmid

    #218017

    Purpose

    mCerulean fluorescent protein

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMBS-871

    Plasmid

    #218019

    Purpose

    8xHis-1xHA-tag for protein detection and purification

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    8xHis-1xHA-tag
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBS-1022

    Plasmid

    #218020

    Purpose

    8xHis-SP20-3xHA-tag for protein detection and purification

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    8xHis-SP20-1xHA-tag
    

    Use

    Synthetic Biology

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge