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Plasmid#226626PurposepiggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2DepositorInsertsUseSynthetic BiologyTagsExpressionMammalianMutationPromoter7xTCF-LEF and EF1aAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCKC764
Plasmid#226625PurposepiggyBAC pCAG-hCas9-T2A-Unbiased3DepositorInsertsCAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, Chicken, S. pyogenes)
EF1a promoter, blasticidin resistance
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV and EF1aAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCKC776
Plasmid#226627PurposepiggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1DepositorInserts4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
EF1a promoter, mCherry
UseSynthetic BiologyTagsoxygen dependent degron (ODD)ExpressionMammalianMutationPromoter4xHRE_YB TATA and EF1aAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human USP7_H456A-3X FLAG
Plasmid#225335PurposeLentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cellsDepositorInsertUSP7 (USP7 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationH456APromoterEF1aAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti-dsRed
Plasmid#201999PurposeBased on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsdsRedExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-U6-GFP-WPRE
Plasmid#200030PurposepiggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDY0551-Luciferase RADARS
Plasmid#182538PurposeMammalian cell expression plasmid of Gaussia luciferase RADARS in a dual luciferase expression back bone with Cypridina luciferase as the control gene.DepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-P-TAG (pGS594)
Plasmid#160039PurposepLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertCP450TMD-APEX2-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-RfxCas13d-Blast-BFP
Plasmid#196726PurposeSortable and selectable RfxCas13d.DepositorInsertRfxCas13d-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsSV40 NLSExpressionMutationPromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TK4-EGFP
Plasmid#239047PurposePiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiationDepositorInsertsEGFP
puroR
UseTagsExpressionMammalianMutationPromoterCAG and EF1aAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only