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Showing: 8851 - 8900 of 12238 results

  2. m.3075-Left TALE-G1397-N-DddA11-mCherry

    Plasmid
    #211337
    Purpose
    Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-DddA11-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211337-plasmid-map-sequence-id-415646

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  3. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  4. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Promoter
    Available since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    137650-plasmid-map-sequence-id-377729

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  5. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

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  6. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  7. pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)

    Plasmid
    #44083
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    44083-plasmid-map-sequence-id-61676

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  8. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid
    #44095
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    44095-plasmid-map-sequence-id-61672

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  9. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  10. pOpen-Exonuclease III E. coli

    Plasmid
    #165571
    Purpose
    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    Exonuclease III E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165571-plasmid-map-sequence-id-340929

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  11. zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)

    Plasmid
    #72638
    Purpose
    Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
    Use
    Gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    72638-plasmid-map-sequence-id-445453

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  12. piggyBAC-BRD4-Flag

    Plasmid
    #214432
    Purpose
    A piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.
    Depositor
    Shaun Cowley
    Insert
    Brd4 bromodomain containing 4 (Brd4 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    214432-plasmid-map-sequence-id-437159

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  13. AAV-CAG-K-SPOTIT

    Plasmid
    #191488
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191488-plasmid-map-sequence-id-377866

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  15. AAV-CAG-M-SPOTIT2

    Plasmid
    #191489
    Purpose
    Green fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-SPOTIT2
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191489-plasmid-map-sequence-id-378568

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  16. pActin5c-CD-dCas9m4-UDI-attB

    Plasmid
    #104879
    Purpose
    To generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9
    Depositor
    Christopher Potter
    Insert
    CD-dCas9(m4)-UDI
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104879-plasmid-map-sequence-id-209447

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  17. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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  18. pTF264 (pFA6a-TEV-6xGly-3xFlag-His3MX)

    Plasmid
    #44071
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44071-plasmid-map-sequence-id-61667

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  19. pTF270 (pFA6a-PSc-6xGly-3xFlag-KanMX)

    Plasmid
    #44089
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44089-plasmid-map-sequence-id-61674

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  20. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

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  21. dClover2-Tubulin-C-18

    Plasmid
    #56376
    Purpose
    Localization: Microtubules, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    dClover2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pTF266 (pFA6a-PSc-6xGly-3xFlag-His3MX)

    Plasmid
    #44065
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44065-plasmid-map-sequence-id-61669

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  24. pTF274 (pFA6a-PSc-6xGly-3xFlag-HphMX)

    Plasmid
    #44077
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44077-plasmid-map-sequence-id-61678

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  25. m.3047-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211339
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3047-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211339-plasmid-map-sequence-id-415656

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  26. m.3075-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211340
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.3075-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211340-plasmid-map-sequence-id-415644

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  27. m.5147-Left TALE-G1397-N-dead-mCherry

    Plasmid
    #211341
    Purpose
    Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection
    Depositor
    Monkol Lek
    Insert
    m.5147-Left TALE-G1397-N-dead-mCherry
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211341-plasmid-map-sequence-id-415657

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  28. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Tara Davis, Melissa Jurica
    Insert
    ppie (PPIE Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  29. PRPF4.001.522.K45A.pc4

    Plasmid
    #137648
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-K45A-pc4
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137648-plasmid-map-sequence-id-381373

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  30. PRPF4.001.522.Y32A.pc4

    Plasmid
    #137646
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-Y32A-pc4
    Promoter
    Available since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137646-plasmid-map-sequence-id-381367

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  31. pNSU299_RPL39

    Plasmid
    #166078
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166078-plasmid-map-sequence-id-330349

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  33. pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)

    Plasmid
    #44073
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44073-plasmid-map-sequence-id-61686

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  34. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Expression
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  35. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  36. pFC36K SmBiT TK-neo Human collagen type IV alpha 3 chain (COL4A3)

    Plasmid
    #229769
    Purpose
    Human Col4A3 tagged at C-terminal with SmBiT to be used with C-tagged LgBiT hCol4A5 and Flag-tagged hCol4A4 in Nanobit assay system
    Depositor
    Hirofumi Kai
    Article
    Omachi et al Cell Chem Biol. 2018 May 17;25(5):634-64
    Insert
    Human collagen type IV alpha 3 chain (COL4A3) (COL4A3 Human)
    Use
    Luciferase
    Tags
    SmBiT
    Expression
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    229769-plasmid-map-sequence-id-456747

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  39. pcDNA-dCas9-p300 Core (D1399Y)

    Plasmid
    #61358
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61358-plasmid-map-sequence-id-99789

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  41. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid
    #208336
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc
    Depositor
    Pranam Chatterjee
    Article
    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:
    Insert
    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    Sc++ (1-1119), SpRY (1111-1368), D10A
    Promoter
    CAG
    Available since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    208336-plasmid-map-sequence-id-439176

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  48. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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  50. pBMN DHFR-HiBiT / Lyt2

    Plasmid
    #179731
    Purpose
    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites
    Depositor
    James Inglese
    Article
    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23
    Insert
    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    Use
    Retroviral
    Tags
    HiBiT
    Expression
    Mutation
    Promoter
    LTR/MMLV
    Available since
    Oct. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    179731-plasmid-map-sequence-id-361671

    Enlarge

Showing: 8851 - 8900 of 12238 results