We narrowed to 2,326 results for: PUC19

1. TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

   Plasmid

   #211906

   Purpose

   Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD22-BBz
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS1528

   Plasmid

   #205982

   Purpose

   U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH DR-BpiI-DR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA scaffold-spacer-human U6

   Plasmid

   #214697

   Purpose

   provide "sgRNA scaffold-spacer-human U6" fragment

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   sgRNA scaffold-spacer-human U6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGP_scFv

   Plasmid

   #183732

   Purpose

   Expresses anti-lysozyme scFv antibody

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Anti-lysozyme scFv
   

   Use

   Tags

   His6-tag

   Expression

   Yeast

   Mutation

   Promoter

   GAP promoter

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

   Plasmid

   #110830

   Purpose

   Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   AZGP1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   T2A and dTomato

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL0747 (pDonor_L-MmeI)

   Plasmid

   #130647

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchCAST donor DNA (L*)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   TGTTGATG --> TGTTGGAG

   Promoter

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEasyG3_nat

   Plasmid

   #184918

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyG3_mic

   Plasmid

   #184920

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PHY98-LMNA 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

   Plasmid

   #164046

   Purpose

   CRISPR donor plasmid to insert TriTag (mTagBFP) into the N-terminus of human LMNA gene

   Depositor

   Baohui Chen

   Article

   Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

   Insert

   mTagBFG-TriTag
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL0746 (pDonor_R-MmeI)

    Plasmid

    #130646

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (R*)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    TGTTGATA --> TGTTGGAA

    Promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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