We narrowed to 159,258 results for: addgene

1. pNR64

   Plasmid

   #79540

   Purpose

   Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   

   Use

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti UBC hNLRP3-mNG

   Plasmid

   #218642

   Purpose

   Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (NLRP3 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TALEN-mROSA26 ELD

   Plasmid

   #60026

   Purpose

   Expression of a TALEN protein specific for mouse ROSA26 locus.

   Depositor

   Radislav Sedláček

   Article

   Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

   Insert

   TALEN-ROSA26
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15xQUAS-TATA

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-I-GFP GX

   Plasmid

   #45443

   Depositor

   John Brigande

   Article

   Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

   Type

   Empty backbone

   Use

   Tags

   IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1α

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pTlpA_mCherry

   Plasmid

   #229146

   Purpose

   Promoter strength evaluation of PtlpA

   Depositor

   Shrikrishnan Sankaran

   Article

   Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No

   Promoter

   PtlpA

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. VV073: 1xCox8 - GCaMP5G in fubi

   Plasmid

   #58509

   Purpose

   Expresses the calcium sensor GCaMP5G in the mitochondria

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   1xCox8 - GCaMP5G
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv2-Stuffer-HepmCherry

   Plasmid

   #192825

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ICP8-P2A/Crimson (pSLIK4)

    Plasmid

    #113859

    Purpose

    For Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-Crimson

    Depositor

    Richard S Myers

    Article

    Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

    Insert

    ICP8
    

    Use

    Lentiviral

    Tags

    P2A/Crimson

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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