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We narrowed to 3,813 results for: biorxiv

Showing: 3561 - 3580 of 3813 results

  3. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

    Plasmid
    #232412
    Purpose
    AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232412-plasmid-map-sequence-id-465038

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  4. MKC175_HBA1reg(synEPOR)

    Plasmid
    #232413
    Purpose
    AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232413-plasmid-map-sequence-id-466145

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  5. A1-LCD allW D262V

    Plasmid
    #234616
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234616-plasmid-map-sequence-id-469043

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  6. A1-LCD allW

    Plasmid
    #234609
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) with all aromatic ami…
    Promoter
    T7
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234609-plasmid-map-sequence-id-469038

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  7. A1-LCD allW D262N

    Plasmid
    #234615
    Purpose
    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    C-terminal domain (186-320) bearing familial ALS …
    Promoter
    T7
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234615-plasmid-map-sequence-id-469028

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  8. p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

    Plasmid
    #229843
    Purpose
    A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Dll4-F2 (exon3)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    229843-plasmid-map-sequence-id-466946

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  10. MKC177_CCR5(PGK-synEPOR)

    Plasmid
    #232415
    Purpose
    AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232415-plasmid-map-sequence-id-465035

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  11. MKC176_EPOR(2A-synEPOR)

    Plasmid
    #232414
    Purpose
    AAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus.
    Depositor
    Kyle Cromer
    Article
    Shah et al bioRxiv 2024.04.12.589260
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232414-plasmid-map-sequence-id-463062

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  12. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid
    #229810
    Purpose
    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    loxp-AmCyan-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229810-plasmid-map-sequence-id-461094

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  13. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

    Plasmid
    #229813
    Purpose
    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    LexA-mODC-LexAOp-cFos
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229813-plasmid-map-sequence-id-460461

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  14. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

    Plasmid
    #229814
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    loxp-EGFP-stop-lox
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229814-plasmid-map-sequence-id-460474

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  15. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

    Plasmid
    #229816
    Purpose
    A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    loxp-EGFP-stop-lox
    Use
    Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229816-plasmid-map-sequence-id-460476

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  17. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid
    #229824
    Purpose
    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Luciferase-P2A-H2A-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229824-plasmid-map-sequence-id-460485

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  18. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid
    #229841
    Purpose
    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    loxp-H2B-mCerulean-2xStop-loxP
    Use
    Cre/Lox; Tol2 based expression vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229841-plasmid-map-sequence-id-461093

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  19. p5E-Crestin-pro (JDW 1320)

    Plasmid
    #229830
    Purpose
    A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Crestin promoter, c-fos minimal promoter, and b-globin intron
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    229830-plasmid-map-sequence-id-458191

    Enlarge

Showing: 3561 - 3580 of 3813 results