We narrowed to 1,629 results for: GUS

Showing: 341 - 360 of 1629 results

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HiFi-nCas9-NG_P2A-mCherry

Plasmid

#219936

Purpose

Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

HiFi-nCas9(D10A/R691A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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apt-ABE8e

Plasmid

#219937

Purpose

Mammalian expression of apt-ABE8e protein (Com_93aa-linker_TadA8e) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Com_93-aa_TadA8e

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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Ai35

Plasmid

#34882

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

CAG-Floxed Archaerhodopsin3-GFP

Use

Mouse Targeting

Tags

GFP

Promoter

CAG

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CROP-seq-copGFP-v2-no-scaffold

Plasmid

#228520

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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Ai39

Plasmid

#34884

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

CAG-Floxed eNpHRv3.0-EYFP

Use

Mouse Targeting

Tags

EYFP

Promoter

CAG

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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apt-ABE8.20

Plasmid

#219938

Purpose

Mammalian expression of apt-ABE8.20 protein (TadA8.20_32aa-linker_Com)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

TadA8.20_32-aa_Com

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Kaptin

Plasmid

#100511

Purpose

Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

KPTN (KPTN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE1-2

Plasmid

#219945

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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nCas9-NG_P2A-mCherry

Plasmid

#219934

Purpose

Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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MOBE4

Plasmid

#219944

Purpose

Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-SL3_PT2A_apt-ABE8.20

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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3'-com-gRNA GG backbone (MOBE1-4)

Plasmid

#219947

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-com-3'end (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-aid-p2a-GFP

Plasmid

#161976

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GSlinker-aid-GFP

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-GFP-p2a-aid

Plasmid

#161975

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GFP-aid-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-blastR-p2a-v5-dL5

Plasmid

#161980

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

blastR-p2a-v5-dL5-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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apt-CBE-3'end

Plasmid

#219939

Purpose

Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

MCP_32-aa_evo-rA1_2xUGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-B2

Plasmid

#67513

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B2 envelope coating virus particle with HiRet by using this plasmid.

Depositor

Kazuto Kobayashi

Article

Kato et al J Neurosci. 2011 Nov 23;31(47):17169-79.

Insert

Fusion Glycoprotein type B2

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Promoter

CAGGS

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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MOBE3

Plasmid

#219943

Purpose

Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-SL3_PT2A_apt-ABE8e

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE3-4

Plasmid

#219946

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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