We narrowed to 8,827 results for: Pol

1. 1203M-CMV-dDiCas7-11-bGHpolyA

   Plasmid

   #220957

   Purpose

   Deactivated DiCas7-11 effector with CMV promoter/enhancer

   Depositor

   Omar Akbari

   Insert

   DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1203P-CMV-Cas7.1-bGHpolyA

   Plasmid

   #220960

   Purpose

   Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector)

   Depositor

   Omar Akbari

   Insert

   Truncated DiCas7-11
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Flag-Jarid2-polyA

   Plasmid

   #223811

   Purpose

   IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

   Depositor

   Azusa Inoue

   Article

   Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

   Insert

   Jarid2 (Jarid2 Mouse)
   

   Use

   In vitro transcription

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest-pol2-Smarcd1-Myc

   Plasmid

   #227716

   Purpose

   Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

   Depositor

   Kent Hunter

   Article

   Ross et al bioRxiv 2024.01.24.577061

   Insert

   Smarcd1 (Smarcd1 Mouse)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   pol2

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDestTol2pA ins:Apollo-NADP+ mVenus

   Plasmid

   #210518

   Purpose

   Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

   Depositor

   Jonathan Rocheleau

   Article

   Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

   Insert

   Apollo-NADP+
   

   Use

   Zebrafish expression, tol2 kit gateway-compatible

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   Zebrafish insulin promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-AN-HA_aa 1-420 POLI

   Plasmid

   #201582

   Purpose

   Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-420 of DNA polymerase iota

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV6-AN-HA_aa 1-449 POLH

   Plasmid

   #201583

   Purpose

   Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   POLH (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-449 of DNA polymerase eta

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV6-AN-HA_aa 1-542 POLK

   Plasmid

   #201585

   Purpose

   Expresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   Polymerase kappa (POLK Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 1-542 of DNA polymerase kap…

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJRM919_WT aa 1-419 His POLI

   Plasmid

   #201445

   Purpose

   Low copy vector for expressing the catalytic domain (amino acids 1-419) of human DNA polymerase iota in E. coli with an N-terminal 6x His tag. C-terminal truncation mutant of pJRM919_WT His POLI

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Expresses amino acids 1-449 of DNA polymerase iot…

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid

    #192582

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid

    #192581

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX4T3-GST-PolB(T304I)

    Plasmid

    #177133

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST tol2 UbB polyA

    Plasmid

    #188701

    Purpose

    Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence

    Depositor

    David Tobin

    Article

    Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-GST-PolB(K72A)

    Plasmid

    #177132

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1C-FlucV5His6-PolyA-HhR_AB

    Plasmid

    #148303

    Purpose

    Insect Expression of PolyA-HhR

    Depositor

    Elisa Izaurralde

    Insert

    PolyA-HhR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1026L=U6-3-gRNA#1[Pol-γ35]

    Plasmid

    #159674

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#1[Pol-γ35]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1026M=U6-3-gRNA#2[Pol-γ35]

    Plasmid

    #159675

    Purpose

    Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    U6-3-gRNA#2[Pol-γ35]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC_cherry donor_Polb target (pE038)

    Plasmid

    #149349

    Purpose

    Replace donor vector for cherry insertion using the human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV6-AN-DDK_RKK/AAA POLI

    Plasmid

    #131240

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert arginine re…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-AN-DDK_3KA + S580A POLI

    Plasmid

    #131241

    Purpose

    Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains point mutations that convert Pol iota se…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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