We narrowed to 18,058 results for: Gateway
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Plasmid#186382PurposeDestination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-Spe4-Kozak-mEos4b-RfA
Plasmid#186380PurposeDestination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mClover3-RfA
Plasmid#186379PurposeDestination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mCherry-RfA
Plasmid#186378PurposeDestination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP9
Plasmid#166111PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2DepositorInsertHta2-sg18 (HTA2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SRM1_3UTR
Plasmid#65866PurposeCarries 3' UTR of SRM1, an RBPMS targetDepositorInsertSRM1
UseTagsExpressionMutationPromoterT7Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P110alpha
Plasmid#40230DepositorInsertP110 alpha (PIK3CA Bovine)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-opti-dsRed
Plasmid#201999PurposeBased on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsdsRedExpressionMammalianMutationPromoterEF1aAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Halotag-stop
Plasmid#186387PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-FLP-PGK-Neo
Plasmid#108544PurposeLentivirus vector expessing Flp under EF1a promoter and neomycin under PGK promoterDepositorInsertsflp recombinase
Neomycin
UseTagsExpressionMutationPromoterEF1apha and PGKAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-PDX1
Plasmid#114304PurposeConstitutive expression of the PDX1 trancription factorDepositorInsertPDX1 (PDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Snaptag-RfA
Plasmid#186385PurposeDestination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-C53A
Plasmid#29419DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationCys53AlaPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-C106D
Plasmid#29413DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationCys106AspPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-SspB-HaloTag-TRIO-DHPH1
Plasmid#176126PurposeHeterodimerization with iLID, Rac1 and RhoG activationDepositorInsertTRIO (TRIO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2K7-RIP-Tomato-EFs-Tk::Sh
Plasmid#114311PurposeTissue-specific expression of the tdTomato living color under the control of the Rat Insulin 2 promoter (RIP)DepositorInserttdTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-D149A
Plasmid#29418DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationAsp149AlaPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-DJ1-E18Q
Plasmid#29404DepositorInsertDJ1 (PARK7 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationGlu18GlnPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-DJ1-E18D
Plasmid#29403DepositorInsertDJ1 (PARK7 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationE18DPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-DJ1-E18N
Plasmid#29407DepositorInsertDJ1 (PARK7 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationGlu18AsnPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DDX53
Plasmid#222240PurposeExpress DDX53 protein in bacteriaDepositorInsertDDX53 (DDX53 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-Halotag-RfA
Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_Wheat_live_Cas9
Plasmid#126880PurposeGateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.DepositorInsertWheat_live_Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
EFR Y877F (17)
Plasmid#102415Purposefluorescent tagging. Plant expression of EFR Y877F (17)DepositorInsertEFR (EFR Mustard Weed)
UseTags6xHis and mgfp5ExpressionPlantMutationY877FPromoter35SAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
EFR Y875F (15)
Plasmid#102414Purposefluorescent tagging. Plant expression of EFR Y875F (15)DepositorInsertEFR (EFR Mustard Weed)
UseTags6xHis and mgfp5ExpressionPlantMutationY875FPromoter35SAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_50_CA_repeat
Plasmid#65869PurposeCarries CA repeats of 50 nucleotides in length, an RBPMS targetDepositorInsertCA repeats 50 nucleotides in lngth
UseTagsExpressionMutationPromoterT7Available sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-E18D
Plasmid#29415DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationGlu18AspPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-E18Q
Plasmid#29417DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationGlu18GlnPromoterAvailable sinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-DJ1-D149A
Plasmid#29406DepositorInsertDJ1 (PARK7 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationAsp149AlaPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DJ1-V5-E18N
Plasmid#29412DepositorInsertDJ1 (PARK7 Human)
UseLentiviralTagsV5ExpressionMammalianMutationGlu18AsnPromoterAvailable sinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1-Cre-PGK-Puro
Plasmid#108543PurposeLentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoterDepositorInsertscre recombinase
Puromycin
UseTagsExpressionMutationPromoterEF1apha and PGKAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Venus-Akaluc
Plasmid#209768PurposeVenus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).DepositorInsertVenus-Akaluc
UseLuciferase; Piggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLIX403-ccdB-Blast
Plasmid#158560PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215010PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCLX-PGK-hCDK4
Plasmid#114314PurposeConstitutive expression of the CDK4 proteinDepositorInsertCDK4 (CDK4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215011PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiUBC CASP1[CARD]-mSG2 (inflammasome reporter)
Plasmid#228044PurposeLentiviral stable Inflammasome speck reporter, designed from PMC9623368.DepositorInsertCASP1[CARD]-mSG2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
IDG_SCN2B_OE_1
Plasmid#161624PurposeOverexpresses SCN2BDepositorInsertSCN2B (SCN2B Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMC3_OE_1
Plasmid#161629PurposeOverexpresses TMC3DepositorInsertTMC3 (TMC3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only