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Addgene

We narrowed to 18,058 results for: Gateway

Showing: 7161 - 7200 of 18058 results
  1. pDEST-Spe4-Kozak-mNeonGreen-RfA

    Plasmid
    #186382
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pDEST-Spe4-Kozak-mEos4b-RfA

    Plasmid
    #186380
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pDEST-Spe4-Kozak-mClover3-RfA

    Plasmid
    #186379
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pDEST-Spe4-Kozak-mCherry-RfA

    Plasmid
    #186378
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. HCP9

    Plasmid
    #166111
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2
    Depositor
    Insert
    Hta2-sg18 (HTA2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pDONR221_SRM1_3UTR

    Plasmid
    #65866
    Purpose
    Carries 3' UTR of SRM1, an RBPMS target
    Depositor
    Insert
    SRM1
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 25, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pLenti4/V5-DEST-P110alpha

    Plasmid
    #40230
    Depositor
    Insert
    P110 alpha (PIK3CA Bovine)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
  8. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pDEST-Spe4-RfA-Halotag-stop

    Plasmid
    #186387
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Halo tag into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pLV-EF1-FLP-PGK-Neo

    Plasmid
    #108544
    Purpose
    Lentivirus vector expessing Flp under EF1a promoter and neomycin under PGK promoter
    Depositor
    Inserts
    flp recombinase
    Neomycin
    Use
    Tags
    Expression
    Mutation
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pCWXPG-UBI-PDX1

    Plasmid
    #114304
    Purpose
    Constitutive expression of the PDX1 trancription factor
    Depositor
    Insert
    PDX1 (PDX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pDEST-Spe4-Kozak-Snaptag-RfA

    Plasmid
    #186385
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. pLenti6-DJ1-V5-C53A

    Plasmid
    #29419
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Cys53Ala
    Promoter
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only
  14. pLenti6-DJ1-V5-C106D

    Plasmid
    #29413
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Cys106Asp
    Promoter
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only
  15. pLV-SspB-HaloTag-TRIO-DHPH1

    Plasmid
    #176126
    Purpose
    Heterodimerization with iLID, Rac1 and RhoG activation
    Depositor
    Insert
    TRIO (TRIO Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
  16. p2K7-RIP-Tomato-EFs-Tk::Sh

    Plasmid
    #114311
    Purpose
    Tissue-specific expression of the tdTomato living color under the control of the Rat Insulin 2 promoter (RIP)
    Depositor
    Insert
    tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pLenti6-DJ1-V5-D149A

    Plasmid
    #29418
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Asp149Ala
    Promoter
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only
  18. pDEST40-DJ1-E18Q

    Plasmid
    #29404
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Glu18Gln
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only
  19. pDEST40-DJ1-E18D

    Plasmid
    #29403
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    E18D
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only
  20. pDEST40-DJ1-E18N

    Plasmid
    #29407
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Glu18Asn
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only
  21. pDEST17-DDX53

    Plasmid
    #222240
    Purpose
    Express DDX53 protein in bacteria
    Depositor
    Insert
    DDX53 (DDX53 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. pDEST-Spe4-Kozak-Halotag-RfA

    Plasmid
    #186377
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  23. pDEST-Spe4-RfA-Snaptag-stop

    Plasmid
    #186395
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjection
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  24. pENTR_Wheat_live_Cas9

    Plasmid
    #126880
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and BpiI sites for sgRNA modules.
    Depositor
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  25. EFR Y877F (17)

    Plasmid
    #102415
    Purpose
    fluorescent tagging. Plant expression of EFR Y877F (17)
    Depositor
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y877F
    Promoter
    35S
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
  26. EFR Y875F (15)

    Plasmid
    #102414
    Purpose
    fluorescent tagging. Plant expression of EFR Y875F (15)
    Depositor
    Insert
    EFR (EFR Mustard Weed)
    Use
    Tags
    6xHis and mgfp5
    Expression
    Plant
    Mutation
    Y875F
    Promoter
    35S
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  27. pDONR221_50_CA_repeat

    Plasmid
    #65869
    Purpose
    Carries CA repeats of 50 nucleotides in length, an RBPMS target
    Depositor
    Insert
    CA repeats 50 nucleotides in lngth
    Use
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only
  28. pLenti6-DJ1-V5-E18D

    Plasmid
    #29415
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Glu18Asp
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
  29. pLenti6-DJ1-V5-E18Q

    Plasmid
    #29417
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Glu18Gln
    Promoter
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only
  30. pDEST40-DJ1-D149A

    Plasmid
    #29406
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Asp149Ala
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only
  31. pLenti6-DJ1-V5-E18N

    Plasmid
    #29412
    Depositor
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Glu18Asn
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only
  32. pLV-EF1-Cre-PGK-Puro

    Plasmid
    #108543
    Purpose
    Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter
    Depositor
    Inserts
    cre recombinase
    Puromycin
    Use
    Tags
    Expression
    Mutation
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  33. pPB-CAG-Venus-Akaluc

    Plasmid
    #209768
    Purpose
    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Insert
    Venus-Akaluc
    Use
    Luciferase; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  34. pLIX403-ccdB-Blast

    Plasmid
    #158560
    Purpose
    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  35. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  36. pCLX-PGK-hCDK4

    Plasmid
    #114314
    Purpose
    Constitutive expression of the CDK4 protein
    Depositor
    Insert
    CDK4 (CDK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  37. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  38. IDG_SCN2B_OE_1

    Plasmid
    #161624
    Purpose
    Overexpresses SCN2B
    Depositor
    Insert
    SCN2B (SCN2B Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  39. IDG_TMC3_OE_1

    Plasmid
    #161629
    Purpose
    Overexpresses TMC3
    Depositor
    Insert
    TMC3 (TMC3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7161 - 7200 of 18058 results