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Plasmid#161817PurposeExpresses ABE-P48R-UGI in mammalian cellsDepositorInsertbpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
UseCRISPRTagsExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
m.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211342PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3047-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211344PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.5147-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTF277 (pFA6a-TEV-6xGly-2xStrep-KanMX)
Plasmid#44094DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF279 (pFA6a-TEV-6xGly-2xStrep-HphMX)
Plasmid#44082DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC13 (pFA6a-TEV-6xGly-V5-HphMX)
Plasmid#44085DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-K-red-SPOTIT
Plasmid#191490PurposeRed fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoterDepositorInsertK-red-SPOTIT
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-M-red-SPOTIT2
Plasmid#191491PurposeRed fluorescent-based opioid sensor for the mu opioid receptor in AAV viral vector under a CAG promoterDepositorInsertM-red-SPOTIT2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTF271 (pFA6a-TEV-6xGly-12xHis-HphMX)
Plasmid#44081DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC11 (pFA6a-TEV-6xGly-V5-KanMX)
Plasmid#44097DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF267 (pFA6a-TEV-6xGly-12xHis-KanMX)
Plasmid#44093DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF275 (pFA6a-TEV-6xGly-2xStrep-His3MX)
Plasmid#44070DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF280 (pFA6a-PSc-6xGly-2xStrep-HphMX)
Plasmid#44076DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-mut
Plasmid#125207PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-mut
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF269 (pFA6a-PSc-6xGly-12xHis-KanMX)
Plasmid#44087DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)
Plasmid#44078DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF278 (pFA6a-PSc-6xGly-2xStrep-KanMX)
Plasmid#44088DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF273 (pFA6a-PSc-6xGly-12xHis-HphMX)
Plasmid#44075DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
Dox human Citron shRNA 1 mKate
Plasmid#155293PurposeLentivirus for doxycycline inducible expression of nontargeting shRNA, mKate marker, based on Addgene 11652DepositorInsertCitron (CIT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZC04 (pFA6a-PSc-6xGly-PrtA-His3MX)
Plasmid#44066DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)
Plasmid#44069DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF276 (pFA6a-PSc-6xGly-2xStrep-His3MX)
Plasmid#44064DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags2xStrep tag, 6xGly linker, ADH1 transcription ter…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC05 (pFA6a-TEV-6xGly-PrtA-KanMX)
Plasmid#44096DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC10 (pFA6a-PSc-6xGly-V5-His3MX)
Plasmid#44067DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)
Plasmid#44090DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC14 (pFA6a-PSc-6xGly-V5-HphMX)
Plasmid#44079DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC07 (pFA6a-TEV-6xGly-PrtA-HphMX)
Plasmid#44084DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, HphM…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF260 (pFA6a-PSc-6xGly-KanMX)
Plasmid#44086DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZC12 (pFA6a-PSc-6xGly-V5-KanMX)
Plasmid#44091DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, KanM…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF265 (pFA6a-PSc-6xGly-12xHis-His3MX)
Plasmid#44063DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags12xHis tag, 6xGly linker, ADH1 transcription term…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
Plasmid#211343PurposeExpression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selectionDepositorInsertm.3075-Right Puro DdCBE-G1397-C- T1413I-GFP
UseTALENTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS WT
Plasmid#174901Purposeexpression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dox human Citron shRNA 1 GFP
Plasmid#155291PurposeLentivirus for doxycycline inducible expression of human Citron shRNA, GFP marker, based on Addgene 11652DepositorInsertCitron (CIT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral human Citron shRNA 1 GFP
Plasmid#155286PurposeLentiviral expression of human CIT shRNA, GFP expression, based on Addgene 12247DepositorInsertCitron (CIT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2-D
Plasmid#125196PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2-D
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF257 (pFA6a-TEV-6xGly-His3MX)
Plasmid#44068DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF258 (pFA6a-PSc-6xGly-His3MX)
Plasmid#44062DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF261 (pFA6a-TEV-6xGly-HphMX)
Plasmid#44080DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF262 (pFA6a-PSc-6xGly-HphMX)
Plasmid#44074DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTF259 (pFA6a-TEV-6xGly-KanMX)
Plasmid#44092DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags(XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…ExpressionMutationPromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro
Plasmid#208646PurposepcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.DepositorTypeEmpty backboneUseCRISPRTagsClawed frog nucleoplasmin NLS, Flag, Puromycin re…ExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax-SpRY
Plasmid#195279PurposeAllows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)
Plasmid#72639PurposeTol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domainDepositorInsertUAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
UseGatewayTagsExpressionMutationStop codon after mCherry- SEE DEPOSITOR COMMENTS …PromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL2020
Plasmid#86709PurposearaBAD based expression vector for inducible protein production in bacterial hostsDepositorTypeEmpty backboneUseTagsHis10 tagExpressionBacterialMutationPromoteraraBADAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJQ200
Plasmid#78496PurposeBase vector for construction of series of suicide plasmids for gene replacement in ProteobacteriaDepositorTypeEmpty backboneUseSuicide vectorTagsExpressionMutationPromoterAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRL692
Plasmid#70284PurposeTn5-based plasmid for transposition in Synechococcus sp. strain PCC 7942DepositorInsertTransposon Tn5-692
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax-SpG
Plasmid#195278PurposeAllows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562) without EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG without EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS AF
Plasmid#174902Purposeexpression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationY306A, Y384FPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Nras61K-Puro
Plasmid#49404PurposeMSCVpuro based vector, expressing Nras mutant 61K.DepositorInsertN Ras K61 (NRAS Human)
UseRetroviralTagsExpressionMammalianMutation61K constitutively activatedPromoterMSCV promotorAvailable sinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDH18
Plasmid#83803PurposeYFP-CFP positive FRET control; his selectionDepositorInsertYFP-CFP
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2016AvailabilityAcademic Institutions and Nonprofits only