We narrowed to 165,343 results for: addgene

1. NLS-tdPCP-GFP

   Plasmid

   #183934

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-GFP
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFSynWSyt1C2AB(80-421)msGFP

   Plasmid

   #209014

   Purpose

   Overexpression of truncated syt1 tagged with GFP in mammalian cells

   Depositor

   Edwin Chapman

   Article

   Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

   Insert

   syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   aa 80-421 only

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX459-sgRNA048_Grb10

   Plasmid

   #232934

   Purpose

   Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Grb10 S. pyogenes)
   

   Expression

   Mammalian

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB3-B-TelR

   Plasmid

   #204992

   Purpose

   Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPerimCh

   Plasmid

   #227013

   Purpose

   Expresses periplasmic mCherry

   Depositor

   Suzan Rooijakkers

   Article

   Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

   Insert

   PelB-mCherry, arabinose inducible cytosolic GFP
   

   Expression

   Bacterial

   Promoter

   rpsM and pBAD

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. B52_puro_empty_gRNA

   Plasmid

   #197557

   Purpose

   sites for cloning two gRNA with puromycin cassette

   Depositor

   Darius Bägli

   Type

   Empty backbone

   Use

   Empty grna backbone with puromycin resistance gene

   Expression

   Mammalian

   Promoter

   U6 for gRNA, CMV for puromycin resistance.

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC_C_PDK1

   Plasmid

   #111672

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   PDK1 (PDK1 Human)
   

   Expression

   Mammalian

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-eDIO-EYFP

   Plasmid

   #104388

   Purpose

   PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

   Depositor

   Volker Busskamp

   Article

   Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

   Insert

   Enhanced Yellow Fluorescent Protein
   

   Use

   Cre/Lox; Piggybac

   Expression

   Mammalian

   Available Since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1 RagA shRNA #1

    Plasmid

    #30319

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagA shRNA (RRAGA Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCK364.306

    Plasmid

    #192642

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mc2 puro EF1a

    Plasmid

    #206221

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MG-155

    Plasmid

    #26529

    Depositor

    David Baltimore

    Article

    O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

    Insert

    miR-155 (MIR155 Human)
    

    Use

    Retroviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pAV-U6+27-Tornado-F30-TAR Variant-1

    Plasmid

    #129406

    Purpose

    circular TAR Variant-1

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-TAR Variant-1
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL WS1.6 WASp

    Plasmid

    #36250

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Promoter

    WS1.6

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 RagA shRNA #2

    Plasmid

    #30320

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagA shRNA (RRAGA Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pT7-SpCas9_sgRNA-site1 (RTW443)

    Plasmid

    #160136

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Expression

    Bacterial

    Promoter

    yibDp

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. N-terminal Halo-XLF

    Plasmid

    #207546

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROSA26-FlpERT2

    Plasmid

    #149438

    Purpose

    ROSA26 mouse targeting vector with tamoxifen-inducible Flp recombinase

    Depositor

    Primo Schaer

    Article

    Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

    Insert

    Flp-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    ERT2

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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