We narrowed to 2,296 results for: pet28

1. pJK693

   Plasmid

   #72382

   Purpose

   Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)

   Depositor

   T. J. Kappock

   Insert

   cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated alanine-260 to threonine

   Promoter

   T7

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. USP08 (2A9U)

   Plasmid

   #25224

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   usp08.hs.0001-0142 (USP8 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Dimerization Domain, contains amino acids 8-138

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pJK285

   Plasmid

   #71551

   Purpose

   Production of Acetobacter aceti 1023 alanine racemase (alr) with N-terminal His tag (H6AaAlr)

   Depositor

   T. J. Kappock

   Article

   Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

   Insert

   alanine racemase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PP-ChK

   Plasmid

   #25307

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CP-PF14_0020: (CHKA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pAAP21

   Plasmid

   #171207

   Purpose

   bacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motif

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   meg-3 (meg-3 Nematode)
   

   Use

   Tags

   8xHIS

   Expression

   Bacterial

   Mutation

   aa545-862, F705A,Y708A,Y713A, N725A

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBH12 (3DB4)

   Plasmid

   #25171

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ubh12.414.617:B9 (UHRF1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains residues 126-285

   Promoter

   Available since

   April 4, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

7. TJP3

   Plasmid

   #25310

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TJP3:G508-L808:cH6 (TJP3 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids G508-L808

   Promoter

   Available since

   April 4, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pJK652

   Plasmid

   #72470

   Purpose

   Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)

   Depositor

   T. J. Kappock

   Insert

   acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated histidine-233 to glycine

   Promoter

   T7

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HAT1

   Plasmid

   #25239

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HAT1_05 (HAT1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pET_mNG2(1-10)_32aalinker_mNG2(11)

    Plasmid

    #82611

    Purpose

    Bacterial expression vector for split mNeonGreen2 screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    split before mutations

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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