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pTEI038

Plasmid

#105353

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor G10-90

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #409 (Sa)

Plasmid

#98134

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #409

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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hIRF-1 gRNA #351 (Sa)

Plasmid

#105283

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

gRNA_hIRF1 promoter #351

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b – E. coli RecE residues 564-868

Plasmid

#104534

Purpose

Expresses E. coli RecE residues 564-868

Depositor

Charles Bell

Article

Zhang et al Structure. 2009 May 13;17(5):690-702. do

Insert

RecE residues 564-866 (C-terminal nuclease domain)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-ATG101-ATG13 HORMA

Plasmid

#99332

Purpose

Mammalian expression of one subcomplex from the Human ULK1 complex

Depositor

James Hurley

Article

Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

Inserts

ATG101 (ATG101 Human)
ATG13 (ATG13 Human)

Tags

GST

Expression

Insect

Promoter

p10 and pH

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-18 (1wa3)

Plasmid

#80123

Purpose

PH-4GS-I3-01-myc-Late1 (1wa3)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-18 (1wa3)

Expression

Bacterial and Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01(ΔL1)

Plasmid

#80049

Purpose

Expression of EPN-01 mutated in TSG101 binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP)

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL2)

Plasmid

#80050

Purpose

Expression of EPN-01 mutated in ALIX binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaYP)

Tags

Myc

Expression

Mammalian

Mutation

YP37AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL1)

Plasmid

#80042

Purpose

Expression of PTAP L domain mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

pCMV-EPN-01*(ΔL2)

Tags

Myc

Expression

Mammalian

Mutation

PTAP sequence in p6 mutated to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL2)

Plasmid

#80043

Purpose

Expression of YPxL L domain mutantd EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

YP37 in p6 mutated to AA

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL1+L2)

Plasmid

#80044

Purpose

Expression of EPN-01* mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPJ173(JPUB_006777)

Plasmid

#83834

Purpose

Carries NEW operon 10 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3336
Kuste3339
Kuste3341

Promoter

op10_3339_for, op10_3341_for, and op10_BsaI_3336_…

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPJ174(JPUB_006779)

Plasmid

#83827

Purpose

Carries operon 1 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3603
kuste3605
kuste3606

Use

Pfab217 (p15a ori, kanr)

Promoter

op1_3603_for, op1_3605_for, and op1_3606_for

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPJ167(JPUB_006765)

Plasmid

#83831

Purpose

Carries NEW operon 3 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste2803
Kuste2804

Promoter

op3_2804_for and op3_BsmBI_2803_for

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a+D77R/T214V

Plasmid

#66135

Purpose

Expresses the D77R/T214V mutant of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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RCAN1-aa1-150

Plasmid

#65412

Purpose

RCAN1 aa1-150 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1-aa1-150 (Rcan1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

aa1-150 present, deleted 151 to end

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB6 Cterminal tail

Plasmid

#60403

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
pVenus-N1 cyclin B1 L45A (1324)

Plasmid

#61851

Purpose

mammalian expression of cyclin B1 L45 A mutant fused to Venus

Depositor

Jonathon Pines

Article

Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

Insert

cyclin B1 (CCNB1 Human)

Tags

Venus

Expression

Mammalian

Mutation

L45A

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-R

Plasmid

#60983

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Promoter

Bactin2

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only