We narrowed to 6,467 results for: poly

1. pYFP-RPB1aAmr

   Plasmid

   #75284

   Purpose

   Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

   Depositor

   Robert Singer, Xavier Darzacq

   Article

   Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   N792D alpha-amanitin–resistant

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINB-AMSH*

   Plasmid

   #66712

   Purpose

   Expresses activated AMSH in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   hbp (Stam2 Mouse)
   AMSH (STAMBP Human)
   

   Use

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. gRNA-trp-HYB

   Plasmid

   #64331

   Purpose

   trp1 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of trp1 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCMV6-AN-HA_WT REV1

   Plasmid

   #201586

   Purpose

   Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA repair protein REV1 (REV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVTAG-NEMO

   Plasmid

   #11970

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. JT113-pETDuet1-(R)-hREV3L

   Plasmid

   #64872

   Purpose

   bacterial expression of human REV3L

   Depositor

   Richard Wood

   Article

   Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

   Insert

   REV3L (REV3L Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOG-Cre

   Plasmid

   #233265

   Purpose

   Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26

   Depositor

   Mauro Calabrese

   Article

   Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM7

   Plasmid

   #154164

   Purpose

   mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-Flag-Rheb

   Plasmid

   #165031

   Purpose

   expression of Flag tagged Rheb protein in mammalian cells

   Depositor

   Ken Inoki

   Article

   Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

   Insert

   Rheb (RHEB Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gRNA-ura-HYB

    Plasmid

    #64330

    Purpose

    ura3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of ura3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pTSara-C

    Plasmid

    #60722

    Purpose

    Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    Residues 180-880 of T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Only amino acids 180-880 of T7 RNAP

    Promoter

    PBAD

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTSara-N

    Plasmid

    #60721

    Purpose

    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    Residues 1-179 of T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Only amino acids 1-179 of T7 RNAP

    Promoter

    PBAD

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-ATXN1

    Plasmid

    #48189

    Purpose

    Expresses Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GAL FLAG103QDProGFP p306

    Plasmid

    #15834

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

15. GAL FLAG103QDProGFPp303

    Plasmid

    #15587

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. GAL FLAG72QDProGFP p306

    Plasmid

    #15833

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pLyGo-Ec-8

    Plasmid

    #163136

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P(rhaB)

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Use

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. GAL FLAG25QDProGFP p306

    Plasmid

    #15831

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pPRIME-TET-GFP-FF3

    Plasmid

    #11662

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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