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pDest17_A1-LCD swap X9

Plasmid

#226551

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X9

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X8

Plasmid

#226550

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X8

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X8

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X6

Plasmid

#226548

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X6

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X6

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X2

Plasmid

#226544

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-L1374

Plasmid

#226275

Purpose

Plasmid expressing the SEC18 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-S288C

Plasmid

#226274

Purpose

Plasmid expressing the SEC18 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-NCYC110

Plasmid

#226277

Purpose

Plasmid expressing the SEC18 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-UWOPS872421

Plasmid

#226276

Purpose

Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-S288C

Plasmid

#226266

Purpose

Plasmid expressing the SCT1 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-L1374

Plasmid

#226267

Purpose

Plasmid expressing the SCT1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-NCYC110

Plasmid

#226269

Purpose

Plasmid expressing the SCT1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-UWOPS872421

Plasmid

#226268

Purpose

Plasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc100_CSC

Plasmid

#210592

Purpose

Expresses lambdaHA-optimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-optimal Firefly luciferase (Dm optimization, CSC metric)

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_Opt_Renilla_CSC

Plasmid

#210593

Purpose

Expresses Optimal Renilla luciferase (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla luciferase (Optimized for Drosophila, CSC metric)

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_OptλHAramp-FLuc100

Plasmid

#210577

Purpose

Expresses Optimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal lambda HA-Optimal Firefly luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_NonoptλHAramp-FLuc100

Plasmid

#210579

Purpose

Expresses Nonoptimal lambda HA-Optimal Firefly luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Nonoptimal lambda HA-Optimal Firefly luciferase

Use

Lentiviral and Luciferase

Tags

lambda HA

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_SM-FLuc100-pA

Plasmid

#210584

Purpose

Expresses SM(FLAG)-optimal Firefly luciferase - pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Dm_λHA-FLuc0_CSC

Plasmid

#210589

Purpose

Expresses lambdaHA-nonoptimal Firefly (CSC metric; Drosophila)

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

LambdaHA-nonoptimal Firefly luciferase (Dm optimization, CSC metric)

Use

Luciferase

Tags

lambda HA

Expression

Insect

Promoter

Ac5

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::acc-1::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225926

Purpose

Expressing ACC-1 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

ACC-1 (acc-1 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37WT-FLAG

Plasmid

#225498

Purpose

Expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37SHPmut_FLAG

Plasmid

#225499

Purpose

Expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-tdc-1P::AI::lgc-47::SL2::his-24::tagRFP::unc-54-3'UTR

Plasmid

#225925

Purpose

Expressing LGC-47 in RIM neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

tdc-1

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3'UTR

Plasmid

#225923

Purpose

Expressing LGC-47 in AIB neuron

Depositor

Andrew Leifer

Article

Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

Insert

LGC-47 (lgc-47 Nematode)

Expression

Worm

Promoter

npr-9

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm8.1

Plasmid

#210288

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.0

Plasmid

#210300

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells with an added 5'UTR

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p8390 LentiCRISPRv2 Neo sgNT-2

Plasmid

#221650

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-2

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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