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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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(P5)pdc

Plasmid

#251385

Purpose

Gene encoding pyruvate decarboxylase (Zymomonas mobilis)

Depositor

Kate Adamala, Pauli Kallio

Publication

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

pdc

Expression

Bacterial

Available since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-GG

Plasmid

#213039

Purpose

AAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)

Depositor

Fredrik Sterky

Publication

De La Cruz et al Bio Protoc. 2024 Nov 5;14(21):e5097. doi

Type

Empty backbone

Use

AAV; Vector for golden gate assembly

Tags

N/A

Promoter

N/A

Available since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Expression

Yeast

Promoter

TEF1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a_DIO_HA/FLAG_muMASS_LF

Plasmid

#213394

Purpose

Loss of function variant of uMASS_1 opioid sensor. AAV virus production for Cre dependent expression.

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.t

Plasmid

#176214

Purpose

Bacterial expression of the SR6v15.t targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Publication

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.t

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15.d

Plasmid

#176213

Purpose

Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Publication

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15.d

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE3G_G

Plasmid

#240261

Purpose

Circuit-responsive (TRE3G promoter) expression of vesicular stomatitis virus G protein (VSVG). Used to produce envelope protein for PROTEUS VLVs during tTA/rtTA-3G campaigns.

Depositor

Gregory Neely

Publication

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

VSV-G

Expression

Mammalian

Promoter

TRE3G

Available since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_CBh

Plasmid

#241376

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with CBh promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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(K) tNOS (S)_T3 (SBE120)

Plasmid

#195010

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(K) tNOS (S)

Expression

Bacterial

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(H) tNOS (I) _T2 (SBE119)

Plasmid

#195006

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(H) tNOS (I)

Expression

Bacterial

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(E) tNOS (F) _T1 (SBE118)

Plasmid

#194997

Purpose

terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(E) tNOS (F)

Expression

Bacterial

Available since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH147

Plasmid

#186647

Purpose

aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA

Depositor

Adam Arkin

Publication

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pPD301 ZF43x16-Compact_EYFP

Plasmid

#138911

Purpose

EYFP reporter vector for the ZF43x16-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x16-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x16-Compact

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD299 ZF43x14-Compact_EYFP

Plasmid

#138909

Purpose

EYFP reporter vector for the ZF43x14-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x14-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x14-Compact

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pPD294 ZF43x10-Compact_EYFP

Plasmid

#138905

Purpose

EYFP reporter vector for the ZF43x10-Compact promoter

Depositor

Joshua Leonard

Insert

ZF43x10-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x10-Compact

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2D'(sfGFP)

Plasmid

#126994

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1D'(sfGFP)

Plasmid

#126981

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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