We narrowed to 4,063 results for: biorxiv

1. MBP-ATG13 (363-517)-ULK1 (836-1050)-His

   Plasmid

   #222824

   Purpose

   Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Tags

   6His and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-GST-ATG13 (363-517)-ULK1 (836-1050)

   Plasmid

   #222822

   Purpose

   Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Tags

   6His-GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP

   Plasmid

   #222821

   Purpose

   Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13, ULK1 (ATG13, ULK1 Human)
   

   Tags

   TwinStrep Flag and msfGFP-10His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFPEasy9R

   Plasmid

   #218291

   Purpose

   Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pToxAmp21R

   Plasmid

   #218287

   Purpose

   A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pToxAmp12

   Plasmid

   #218286

   Purpose

   Multi-copy integration of heterologous genes (AeBlue and RelB) through co-transformation with ToxAmp (toxin-antitoxin-driven gene amplification) modules

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-pCRT1>RelB>tPDC1-pTDH3>AeBlue>tsynth7-ARS712-HO(-731, -264)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pToxAmp1

   Plasmid

   #218285

   Purpose

   Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pToxamEXP4CUP1

   Plasmid

   #218284

   Purpose

   Use together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   Hph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV2_CAG_oROS-HT(C199S)_WPRE

   Plasmid

   #216415

   Purpose

   Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT(C199S)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-FKBP-GFP-NAP1-S37K/A44E (delta-NDP52)

    Plasmid

    #208863

    Purpose

    Stably express GFP-tagged NAP1 (delta-NDP52) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    S37K/A44E (disrupts NDP52 binding)

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-FKBP-GFP-NAP1-I11S/L12S (delta-FIP200)

    Plasmid

    #208864

    Purpose

    Stably express GFP-tagged NAP1 (delta-FIP200) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    I11S/L12S (disrupts FIP200 binding)

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-FKBP-GFP-NAP1-L226Q/L233Q (delta-TBK1)

    Plasmid

    #208865

    Purpose

    Stably express GFP-tagged NAP1 (delta-TBK1) for CID induced mitophagy

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Mutation

    L226Q/L233Q (disrupts TBK1 binding)

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid

    #208399

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid

    #208398

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    E2-Crimson

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid

    #208400

    Purpose

    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Baptiste Pradel et al bioRxiv

    Insert

    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p10xUAS-WHaloCaMP1a-EGFP

    Plasmid

    #205314

    Purpose

    Expression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Expression

    Insect

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-WHaloCaMP-eNOSpep-EGFP

    Plasmid

    #205306

    Purpose

    Expression of WHaloCaMP-eNOSpep-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP-eNOSpep-EGFP
    

    Expression

    Bacterial

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF

    Plasmid

    #203549

    Purpose

    Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13, ULK1 (ATG13, ULK1 Human)
    

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)

    Plasmid

    #203548

    Purpose

    Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13, ULK1 (ATG13, ULK1 Human)
    

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)

    Plasmid

    #203547

    Purpose

    Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13, ULK1 (ATG13, ULK1 Human)
    

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge