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We narrowed to 9,801 results for: pET

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Showing: 3721 - 3740 of 9801 results

  3. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid
    #117911
    Purpose
    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM
    Depositor
    Kai Johnsson
    Article
    Yu et al Science. 2018 Sep 14;361(6407):1122-1126
    Insert
    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    R44A & H45Q on eDHFR
    Promoter
    T7
    Available Since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    117911-plasmid-map-sequence-id-226235

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  6. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid
    #101719
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101719-plasmid-map-sequence-id-199092

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  9. pET28a SUMO Nup53xl 162-267

    Plasmid
    #86002
    Purpose
    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53
    Depositor
    Wolfram Antonin
    Type
    Empty backbone
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86002-plasmid-map-sequence-id-171435

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  11. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available Since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  17. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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Showing: 3721 - 3740 of 9801 results