We narrowed to 17,198 results for: puro

1. CLK4 gRNA (BRDN0001146113)

   Plasmid

   #77798

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CLK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CLK4 (CLK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ARSG gRNA (BRDN0001147989)

   Plasmid

   #77640

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ARSG

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ARSG (ARSG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GUCY2F gRNA (BRDN0001147943)

   Plasmid

   #77254

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human GUCY2F

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   GUCY2F (GUCY2F Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CAMK4 gRNA (BRDN0001145862)

   Plasmid

   #77226

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CAMK4

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CAMK4 (CAMK4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ANKK1 gRNA (BRDN0001149290)

   Plasmid

   #76035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ANKK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   ANKK1 (ANKK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-(-)-Pur

   Plasmid

   #200458

   Purpose

   Eukaryotic expression vector

   Depositor

   Bertrand Collet

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-Pur-mEGFP

   Plasmid

   #186329

   Purpose

   Fluorescent reporter system

   Depositor

   Bertrand Collet

   Insert

   mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TET-PH-lox66FRT

   Plasmid

   #139561

   Purpose

   piggyBac expression vector with TET-ON system and puromycin selection

   Depositor

   Minoru Ko

   Insert

   PGK-puroR-pA cassette, inserted in PB-TET vector
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 7TFP

   Plasmid

   #24308

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-FFluc // SV40-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKLV-gRNA-CTBP2-MGMT_1

    Plasmid

    #136419

    Purpose

    Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

    Depositor

    Massimo Squatrito

    Insert

    U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKLV-gRNA-BTRC-MGMT_2

    Plasmid

    #136416

    Purpose

    Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

    Depositor

    Massimo Squatrito

    Insert

    U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. cASIC1 concatemer - vector backbone

    Plasmid

    #233132

    Purpose

    To create cASIC1 channels of defined stoichiometry in mammalian cells

    Depositor

    David MacLean

    Article

    Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 7TGP

    Plasmid

    #24305

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-PuroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NK802

    Plasmid

    #166056

    Purpose

    Puromycin resistance and mCherry expression for Salpingoeca rosetta

    Depositor

    Nicole King

    Article

    Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

    Insert

    Pac puromycin resistance gene
    

    Use

    S. rosetta expression

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. EdTP

    Plasmid

    #24311

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NK803

    Plasmid

    #166057

    Purpose

    Puromycin resistance and LifeAct-mCherry expression for Salpingoeca rosetta

    Depositor

    Nicole King

    Article

    Brunet et al Elife. 2021 Jan 15;10. pii: 61037. doi:

    Inserts

    Pac puromycin resistance gene
    LifeAct-mCherry
    

    Use

    S. rosetta expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJA291

    Plasmid

    #74487

    Purpose

    eF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFP

    Depositor

    Andrew Fire

    Article

    Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

    Insert

    puroR-mCherry-T2A-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pERB122

    Plasmid

    #58282

    Purpose

    const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB

    Depositor

    Michael Lampson

    Article

    Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

    Inserts

    FKBP-targeted miRNA
    GFP
    Puromycin resistance
    inducible mCherry-FRB
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML60

    Plasmid

    #206998

    Purpose

    Contains mAID-tag, mouse Ptbp1-specific homology arms and puromycin resistance gene.

    Depositor

    Eugene Makeyev

    Article

    Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

    Insert

    PuroR
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-DRGFP

    Plasmid

    #113193

    Purpose

    DR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.

    Depositor

    Adam Karpf

    Insert

    DR-GFP
    

    Use

    Donor construct with t2a-hygromycin for gene trap…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Launchpad (AVA2590)

    Plasmid

    #85442

    Purpose

    Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    FRT-flanked EF1alfa-GFP PGK-Puromycin cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

24. lentiCRISPR v2

    Plasmid

    #52961

    Purpose

    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Inserts

    Cas9
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCL1032

    Plasmid

    #217962

    Purpose

    Mammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.

    Depositor

    Andrew Bowman

    Article

    Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

    Insert

    NASP (NASP Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJ151-HDR

    Plasmid

    #113631

    Purpose

    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

    Depositor

    Alex Kentsis

    Inserts

    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    

    Use

    Cre/Lox; Homology recombination selection vector

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a promoter, EF1a promoter (T2A cleaving sequen…

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSBbi-TIR1-tTA/Pur

    Plasmid

    #171683

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Inserts

    tetracycline-controlled transactivator
    Puromycin
    EF-1a promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a promoter and RPBSA promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. attB53-Pac-attB53

    Plasmid

    #183610

    Purpose

    RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    Pac
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. cASIC1 concatemer - fully assembled

    Plasmid

    #233133

    Purpose

    To create cASIC1 channels of defined stoichiometry in mammalian cells

    Depositor

    David MacLean

    Article

    Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

    Insert

    ASIC1 (ASIC1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. cASIC1 concatemer - Subunit C

    Plasmid

    #233131

    Purpose

    To create cASIC1 channels of defined stoichiometry in mammalian cells

    Depositor

    David MacLean

    Article

    Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

    Insert

    ASIC1 (ASIC1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. cASIC1 concatemer - Subunit B

    Plasmid

    #233130

    Purpose

    To create cASIC1 channels of defined stoichiometry in mammalian cells

    Depositor

    David MacLean

    Article

    Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

    Insert

    ASIC1 (ASIC1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. cASIC1 concatemer - Subunit A

    Plasmid

    #233129

    Purpose

    To create cASIC1 channels of defined stoichiometry in mammalian cells

    Depositor

    David MacLean

    Article

    Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

    Insert

    ASIC1 (ASIC1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPB-tetpA(R1R4)-iRPT

    Plasmid

    #154882

    Purpose

    Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRosetta_v2

    Plasmid

    #136477

    Purpose

    Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

    Insert

    HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PX459-sgRNA048_Grb10

    Plasmid

    #232934

    Purpose

    Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    Cas9-T2A-PuroR (Grb10 S. pyogenes)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. E[beta]P

    Plasmid

    #24313

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-betacatenin(deltaGSK) // SV40-PuroR (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Sites phosphorylated by GSK-3beta have been mutat…

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMP472

    Plasmid

    #134997

    Purpose

    Interspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    Inters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMP498

    Plasmid

    #134998

    Purpose

    Clustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    Clust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge