We narrowed to 10,589 results for: kars;

1. SIN40C.SFFV.dTomato.let-7c

   Plasmid

   #169301

   Purpose

   Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   let-7c (MIRLET7C Human)
   

   Use

   Lentiviral

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. hHSF1 K80Q

   Plasmid

   #118351

   Purpose

   Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

   Depositor

   Lea Sistonen

   Article

   Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K80Q

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT371 PB ITR and Cp36 attD pEF Puro-2a-mCherry donor

   Plasmid

   #184951

   Purpose

   Donor for both PiggyBac and Cp36 Recombinase

   Depositor

   Patrick Hsu, Ami Bhatt, Michael Bassik, Lacra Bintu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   PuroR, P2A, mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBMN-HA-TBK1

   Plasmid

   #199205

   Purpose

   Used for the expression of TBK1 (SMC Internal No. 1996)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIC-myc-GBAi W211A

   Plasmid

   #171754

   Purpose

   For the mammalian expression of myc-GBAi W211A

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD-T2A-split-intein-Gal4[1-20]-N-int

   Plasmid

   #199203

   Purpose

   To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

   Depositor

   Norbert Perrimon

   Article

   Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

   Insert

   T2A-split-intein-Gal4[1-20]-N-int
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EF1a_SPI1_P2A_Hygro_Barcode

   Plasmid

   #120482

   Purpose

   Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SPI1 (SPI1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI

   Plasmid

   #226009

   Purpose

   DjCas13d guide RNA cloning backbone (lenti)

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERH-ZNF281

   Plasmid

   #222923

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of ZNF281

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   ZNF281 (ZNF281 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. amilCP_Pink chromoprotein

    Plasmid

    #117851

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP_Pink
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    64A,65C, BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid

    #172506

    Purpose

    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 bacterial expression vector with golden gate site for guides
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. gfasPurple chromoprotein

    Plasmid

    #117849

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, gfasPurple
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 6691 Bicistronic_ires_puro

    Plasmid

    #64335

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSMP-EZH2_1

    Plasmid

    #36387

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    EZH2 (EZH2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p27-IRES-mCherry

    Plasmid

    #80143

    Purpose

    retroviral expression of p27 and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    p27 (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBP-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199202

    Purpose

    Gateway destination vector for enhancer-driven split-intein Gal4[21-881]C-int expression

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge