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pET28 MBP-TEV-Rat BCKDK-His

Plasmid

#232119

Purpose

For bacterial expression of His-tagged Rat BCKDK (AA31-412, missing precursor peptide) with a cleavable MBP purification tag.

Depositor

Ruth Sommese

Article

Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

Insert

BCKDK (Bckdk Rat)

Tags

6 x His, Maltose-Binding Protein (MBP), and Tev S…

Expression

Bacterial

Promoter

T7 Promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-HCN3 [N141/28R-2b]

Plasmid

#219484

Purpose

Mammalian Expression Plasmid of anti-HCN3 ion channel (Mouse) subclass-switched IgG2b R-mAb. Derived from hybridoma N141/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-HCN3 ion channel (Mus musculus) recombinant mouse monoclonal antibody. (Hcn3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-TMEM240 [N498/28R]

Plasmid

#220417

Purpose

Mammalian Expression Plasmid of anti-TMEM240 (Human) IgG2a R-mAb. Derived from hybridoma N498/28

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-TMEM240 (Homo sapiens) recombinant mouse monoclonal antibody. (C1orf70 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6p1_RBFOX2_RRM

Plasmid

#224293

Purpose

GST-RBFOX2(RRM domain) purification from bacteria

Depositor

Chuan He

Article

Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

Insert

RNA binding fox-1 homolog 2 RNA-recognition motif (RBFOX2 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 74-167

Promoter

tac promoter

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS

Plasmid

#209787

Purpose

Expresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

TNT4, TadA8e, SpRY N, inteinN, miR122 TS

Use

AAV

Promoter

TNT4

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GRK6A/B [N142/28R]

Plasmid

#206533

Purpose

Mammalian Expression Plasmid of anti-GRK6A/B (Mouse). Derived from hybridoma N142/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GRK6A/B (Rattus norvegicus) recombinant Mouse monoclonal antibody (Grk6 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pITGA5_1745G>U

Plasmid

#173812

Purpose

mir218 binding site2 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT1745 ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_473G>A

Plasmid

#173811

Purpose

mir218 binding site1 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT473 ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR218-2_nt5G>U

Plasmid

#174009

Purpose

miR218 nt5 point mutation in SLIT3

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

SLIT3 (SLIT3, MIR218-2 Human)

Expression

Mammalian

Mutation

NT5 G>U point mutation in SLIT3

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_Q853L

Plasmid

#174008

Purpose

Fibronectin receptor binding site point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

FibronR ITGA5 point mutation (ITGA5 Human)

Expression

Mammalian

Promoter

AmpR

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-PSD-93/Chapsyn-110 [N18/28R]

Plasmid

#177505

Purpose

Mammalian Expression Plasmid of anti-PSD-93/Chapsyn-110 (Rat). Derived from hybridoma N18/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-PSD-93/Chapsyn-110 (Rattus norvegicus) recombinant mouse monoclonal antibody (Dlg2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

Plasmid

#173986

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #2

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-EC1.45-human_min1-opossum_min2

Plasmid

#173966

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

See Depositor Comments Below

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

Plasmid

#173994

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated

Use

Luciferase

Mutation

7X Coordinator motif mutation #1-2-3-4-5-6-7

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_4XCoordinatorMutant

Plasmid

#173962

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 4 mutated Coordinator motifs

Use

Luciferase

Mutation

4 mutated Coordinator motifs

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#1

Plasmid

#173985

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #1 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #1

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#3

Plasmid

#173987

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #3 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #3

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#5

Plasmid

#173989

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #5 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #5

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_4XCoordinatorMutant#1-2-3-4

Plasmid

#173992

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motifs #1, #2, #3 and #4 mutated

Use

Luciferase

Mutation

4X Coordinator motif mutations #1-2-3-4

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#6

Plasmid

#173990

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #6 mutated

Use

Luciferase

Mutation

Coordinator motif mutation #6

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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