We narrowed to 4,353 results for: erf

1. AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

   Plasmid

   #222107

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-mCherry

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAVS1_dCas9-XTEN-KRAB-p2A-GFP

   Plasmid

   #222108

   Purpose

   Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

   Depositor

   Jonathan Weissman

   Article

   She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

   Insert

   CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
   

   Use

   AAV and CRISPR

   Tags

   HA and P2A-GFP

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. USP7 shRNA-TRE

   Plasmid

   #225338

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   USP7 shRNA (Usp7 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (tetracycline-responsive element)

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Luciferase shRNA-TRE

   Plasmid

   #225339

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   Luciferase shRNA (LOC116160065 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (tetracycline-responsive element)

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMBS-856

   Plasmid

   #218014

   Purpose

   sfGFP fluorescent protein

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GL4.10 E2E1- Prg4 promoter-luciferase

   Plasmid

   #195035

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.10 E3E2E1-Prg4 promoter-luciferase

   Plasmid

   #195036

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

   Plasmid

   #195037

   Purpose

   Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMM7

   Plasmid

   #201213

   Purpose

   Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

   Depositor

   Alex Nielsen

   Article

   Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

   Inserts

   sfGFP
   Spo0A right flank
   Spo0A left flank
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   F11V, T13P, N39D, A179A

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJL1-sfGFP-MOORmut

    Plasmid

    #198213

    Purpose

    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    sfGFP_MOORmut
    

    Use

    Tags

    6x His Tag and mutated (non-permissible) MOORmut …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLiP-G1G2

    Plasmid

    #188965

    Purpose

    L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

    Depositor

    Seung-Goo Lee

    Article

    Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

    Inserts

    dCas9
    sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pST_CAHS1_PARRC_150-189 (pBS0658)

    Plasmid

    #185201

    Purpose

    For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS1_PARRC_150-189
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pST_APOE_HUMAN_A234G (pBS0816)

    Plasmid

    #185267

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_A234G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A234G

    Promoter

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST_D4NWF2_DrHD_b03_dom3 (pBS0681)

    Plasmid

    #185207

    Purpose

    For the mammalian expression of the Deinococcus radiodurans protein D4NWF2_DrHD_b03_dom3. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    D4NWF2_DrHD_b03_dom3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST_AfLEA1.1 (pBS0741)

    Plasmid

    #185233

    Purpose

    For the mammalian expression of the brine shrimp protein AfLEA1.1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfLEA1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pST_Q19228_CAEEL_51-88 (pBS0734)

    Plasmid

    #185230

    Purpose

    For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-88. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    Q19228_CAEEL_51-88
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pST_pvLEA22mer_shuffle_5 (pBS0731)

    Plasmid

    #185228

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pST_pvLEA22mer_shuffle_3 (pBS0729)

    Plasmid

    #185227

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST_pvLEA22mer_shuffle_1 (pBS0727)

    Plasmid

    #185226

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pST_pvLEA22mer_mutant_5 (pBS0722)

    Plasmid

    #185225

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_5. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge