We narrowed to 3,807 results for: golden gate

1. pMB.PRC2.AEBP2

   Plasmid

   #234580

   Purpose

   Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4 and AEBP2 [Isoform 3]

   Depositor

   Chen Davidovich

   Article

   Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

   Insert

   EZH2, SUZ12, EED, RBBP4, AEBP2 [Iso 3] (EZH2, SUZ12, RBBP4, EED, AEBP2 Human)
   

   Expression

   Insect

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGD_RT_li_Venus

   Plasmid

   #136973

   Purpose

   Goldengate/Greengate C-tag module, containing Venus fused to a flexible linker

   Depositor

   Klaus Theres

   Article

   Lopez et al Plant Cell. 2021 Aug 13;33(7):2412-2430.

   Insert

   Venus
   

   Expression

   Bacterial

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGGF_RT_FAST_RFP

   Plasmid

   #136979

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Article

   Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

   Insert

   seed coat marker RFP
   

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Promoter

   tRNA-Gln

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ttHsCas12a

   Plasmid

   #182385

   Purpose

   Gives high editing efficiency in barley.

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   ttHsCas12a
   

   Use

   CRISPR; Coding sequence only

   Mutation

   D156R

   Promoter

   None

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSAR

   Plasmid

   #62292

   Purpose

   docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDSAP

   Plasmid

   #62293

   Purpose

   docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDSAY

   Plasmid

   #62291

   Purpose

   docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDSAG

   Plasmid

   #62289

   Purpose

   docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDSARN

    Plasmid

    #62295

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDSAT

    Plasmid

    #62290

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDSAYN

    Plasmid

    #62294

    Purpose

    docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSKB-

    Plasmid

    #62540

    Purpose

    Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. JME4580

    Plasmid

    #129659

    Purpose

    HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAGT9403

    Plasmid

    #192352

    Purpose

    L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-tNOS_CGCT
    

    Expression

    Plant

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEED-mScarlet3

    Plasmid

    #219640

    Purpose

    Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEED-ALFA:HA

    Plasmid

    #219646

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    ALFA:HA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEED-sfGFP:ALFA

    Plasmid

    #219642

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEED-mGreenLantern:ALFA

    Plasmid

    #219644

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern:ALFA SEED Cassette
    

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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