Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 2,967 results for: 623

Showing: 541 - 570 of 2967 results

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mIFP-PDHA1-N-10

Plasmid

#56236

Purpose

Localization: Mitochondrial Matrix, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

PDHA1 (PDHA1 Human)

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CL20_mEGFP_FOidr_071

Plasmid

#244623

Purpose

Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_071, derived from human fusion protein HEY1_NCOA2

Depositor

Richard Kriwacki

Article

Maiti et al Sci Adv. 2025 Dec 5;11(49):eady1420. doi

Insert

FOidr_071

Tags

monomeric EGFP

Expression

Mammalian

Mutation

Unmutated

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(WT)-EGFP

Plasmid

#196233

Purpose

EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(WT)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(G42E)-myc

Plasmid

#196238

Purpose

Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(G42E)-myc

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_C74_eMagAF_V5_NES

Plasmid

#235636

Purpose

Encodes fusion protein of ALIBi C-terminal fragment (residues 74-321), eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

eMagAF-V5-NES

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N256_eMagBF_V5_NES

Plasmid

#235630

Purpose

Encodes ALIBi N-terminal fragment, residues 1-256, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-256

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C233

Plasmid

#235629

Purpose

Encodes ALIBi C-terminal fragment, residues 233-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 233-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C141

Plasmid

#235621

Purpose

Encodes ALIBi C-terminal fragment, residues 141-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 141-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C222

Plasmid

#235627

Purpose

Encodes ALIBi C-terminal fragment, residues 222-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 222-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C196

Plasmid

#235625

Purpose

Encodes ALIBi C-terminal fragment, residues 196-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 196-321

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N232_eMagBF_V5_NES

Plasmid

#235628

Purpose

Encodes ALIBi N-terminal fragment, residues 1-232, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-232

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_MyrPalm_NES_V5_eMagAF_C74

Plasmid

#235639

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, MyrPalm for targeting to plasma membrane

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

MyrPalm-NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_V5_eMagAF_C74_cMycNLS

Plasmid

#235640

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, cMyc nuclear localization sequence for targeting to nucleus

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

V5-eMagAF and cMycNLS

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N140_eMagBF_V5_NES

Plasmid

#235620

Purpose

Encodes ALIBi N-terminal fragment, residues 1-140, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-140

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C74_Sec61b

Plasmid

#235637

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, nuclear export sequence, Sec61b for targeting ALIBi to endoplasmic reticulum

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF and Sec61b

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_V5_Fbl_eMagAF_C74

Plasmid

#235641

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, Fbl for targeting to nucleolus

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

V5-Fbl-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C66

Plasmid

#235613

Purpose

Encodes ALIBi C-terminal fragment, residues 66-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 66-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N221_eMagBF_V5_NES

Plasmid

#235626

Purpose

Encodes ALIBi N-terminal fragment, residues 1-221, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-221

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C100

Plasmid

#235619

Purpose

Encodes ALIBi C-terminal fragment, residues 100-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 100-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C257

Plasmid

#235631

Purpose

Encodes ALIBi C-terminal fragment, residues 257-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 257-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_GFP_FTA

Plasmid

#235633

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

FLAG-TEV cleavage site-AviTag and NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C78

Plasmid

#235617

Purpose

Encodes ALIBi C-terminal fragment, residues 78-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 78-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagAF_C74

Plasmid

#235615

Purpose

Encodes ALIBi C-terminal fragment, residues 74-321, fused to eMagAF (enhanced Magnet, acidic, fast), V5, and nuclear export sequence

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi C-terminal fragment

Tags

NES-V5-eMagAF

Expression

Mammalian

Mutation

residues 74-321

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_NES_V5_eMagBF_N73

Plasmid

#235635

Purpose

Encodes fusion protein of nuclear export sequence, V5, eMagBF (enhanced Magnet, basic, fast), ALIBi N-terminal fragment (residues 1-73) (order reversed)

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

NES-V5-eMagBF

Expression

Mammalian

Mutation

residues 1-73

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N167_eMagBF_V5_NES

Plasmid

#235622

Purpose

Encodes ALIBi N-terminal fragment, residues 1-167, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-167

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGEN_N195_eMagBF_V5_NES

Plasmid

#235624

Purpose

Encodes ALIBi N-terminal fragment, residues 1-195, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

ALIBi N-terminal fragment

Tags

eMagBF-V5-NES

Expression

Mammalian

Mutation

residues 1-195

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-9

Plasmid

#228968

Purpose

For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-10

Plasmid

#228969

Purpose

For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-12

Plasmid

#228970

Purpose

For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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