We narrowed to 17,199 results for: puro
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Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GRET
Plasmid#158222PurposeControl lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.DepositorInsertGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1
Plasmid#125840Purposeexpress wild type BAP1DepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PCNA
Plasmid#215117PurposeExpresses mCherry-PCNA in mammalian cells from a lentiviral vector.DepositorInsertPCNA (PCNA Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-C91G
Plasmid#125845Purposeexpress mutant BAP1-C91GDepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsFlagExpressionMutationC91GPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-MPro-eGFP-2
Plasmid#184824PurposeExpresses Mpro-eGFP in mammalian cellsDepositorInsertMpro-eGFP conjugate (M SARS-CoV-2, jellyfish Aequorea Victoria)
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alfaAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA1
Plasmid#98399PurposeLentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
EF1a-L274GMmMetRS-T2A-mCherry
Plasmid#220800PurposeLentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistanceDepositorInsertMars1 (Mars1 Mouse)
UseLentiviralTags2xFLAG and T2A-mCherryExpressionMutationmutation in MetRS to change aa 274 from L to GPromoterEF1aAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Mito-Car-GECO
Plasmid#100765PurposeLentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imagingDepositorInsertLenti-Mito-Car-GECO
UseLentiviralTagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1: E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-MA4-GFP
Plasmid#114380PurposeDoxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.DepositorInsertMLL-AF4 fusion gene (AFF1 Human)
UseTagsGFPExpressionMammalianMutationPromoterTREAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP185 pLVP-dCas9-DNMT3a V2
Plasmid#100936PurposedCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backboneDepositorInsertdCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
UseCRISPR and LentiviralTags3xHA and 3xTy1ExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-C91A
Plasmid#125841Purposeexpress mutant BAP1-C91ADepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsFlagExpressionMutationC91APromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAGI1 gRNA (BRDN0001146833)
Plasmid#78067Purpose3rd generation lentiviral gRNA plasmid targeting human MAGI1DepositorInsertMAGI1 (MAGI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAGI1 gRNA (BRDN0001487062)
Plasmid#78068Purpose3rd generation lentiviral gRNA plasmid targeting human MAGI1DepositorInsertMAGI1 (MAGI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAGI1 gRNA (BRDN0001146934)
Plasmid#78069Purpose3rd generation lentiviral gRNA plasmid targeting human MAGI1DepositorInsertMAGI1 (MAGI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAGI1 gRNA (BRDN0001147011)
Plasmid#78070Purpose3rd generation lentiviral gRNA plasmid targeting human MAGI1DepositorInsertMAGI1 (MAGI1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe K-Ras 12V
Plasmid#12544DepositorInsertK-Ras 12V (KRAS Human)
UseRetroviralTagsExpressionMammalianMutation12V constituitively activatedPromoterAvailable sinceSept. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001146057)
Plasmid#76376Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rInsp-hIns-GLuc
Plasmid#89927PurposeExpresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.DepositorInserthuman insulin-Gaussia-Luciferase (INS Human, Synthetic)
UseLentiviralTagsExpressionMammalianMutationDNA sequence for Gaussia luciferase was humanized…Promoter410bp rat insulin II promoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
LMP AMPK alpha1 hp1
Plasmid#89489PurposeshRNA against AMPK alpha1 in mouse/human to knock down protein expressionDepositorUseMouse Targeting and RetroviralTagsExpressionMammalianMutationPromoterU6 (The GFP-Puro is from a PGK promoter; the shRN…Available sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148320)
Plasmid#76378Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
ERBB2 gRNA (BRDN0001148673)
Plasmid#76377Purpose3rd generation lentiviral gRNA plasmid targeting human ERBB2DepositorInsertERBB2 (ERBB2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 1-480 WT
Plasmid#158541PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletionPromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABL1 gRNA (BRDN0001147582)
Plasmid#77732Purpose3rd generation lentiviral gRNA plasmid targeting human ABL1DepositorInsertABL1 (ABL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAPK1 gRNA (BRDN0001149093)
Plasmid#77788Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
px459-AMBRA1 #3
Plasmid#172606PurposeExpresses gRNA #3 against AMBRA1 and Cas9 from S. pyogenes with 2A-PuroDepositorInsertgRNA against AMBRA1 (AMBRA1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
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MAPK1 gRNA (BRDN0001147355)
Plasmid#77787Purpose3rd generation lentiviral gRNA plasmid targeting human MAPK1DepositorInsertMAPK1 (MAPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
HK2 gRNA (BRDN0001162212)
Plasmid#76310Purpose3rd generation lentiviral gRNA plasmid targeting human HK2DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP2K4 gRNA (BRDN0001145028)
Plasmid#76650Purpose3rd generation lentiviral gRNA plasmid targeting human MAP2K4DepositorInsertMAP2K4 (MAP2K4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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PRKDC gRNA (BRDN0001146589)
Plasmid#77861Purpose3rd generation lentiviral gRNA plasmid targeting human PRKDCDepositorInsertPRKDC (PRKDC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
AKT3 gRNA (BRDN0001146868)
Plasmid#76217Purpose3rd generation lentiviral gRNA plasmid targeting human AKT3DepositorInsertAKT3 (AKT3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DDR1 gRNA (BRDN0001162484)
Plasmid#78011Purpose3rd generation lentiviral gRNA plasmid targeting human DDR1DepositorInsertDDR1 (DDR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
DDR1 gRNA (BRDN0001162509)
Plasmid#78012Purpose3rd generation lentiviral gRNA plasmid targeting human DDR1DepositorInsertDDR1 (DDR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-Reverse
Plasmid#22208DepositorUseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
PRKDC gRNA (BRDN0001149021)
Plasmid#77864Purpose3rd generation lentiviral gRNA plasmid targeting human PRKDCDepositorInsertPRKDC (PRKDC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-H169Q
Plasmid#125847Purposeexpress mutant BAP1-H169QDepositorInsertBAP1 (BRCA1 associated protein 1) (BAP1 Human)
UseLentiviralTagsFlagExpressionMutationH169QPromoterAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PRKDC gRNA (BRDN0001149438)
Plasmid#77863Purpose3rd generation lentiviral gRNA plasmid targeting human PRKDCDepositorInsertPRKDC (PRKDC Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes 1-480 188/218-mut
Plasmid#158542PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant EJC-binding deficient CASC3 1-480DepositorInsertCASC3; BTZ; MLN51 (CASC3 Human)
UseTagsV5ExpressionMammalianMutationAmino acids 1-480, C-terminal deletion, F188D, W2…PromoterCMVAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only