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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 159,258 results for: Gene

Showing: 9501 - 9550 of 159258 results

  1. pSO9

    Plasmid
    #106366
    Purpose
    To insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the intestine.
    Depositor
    Jonathan Ewbank
    Article
    Lee et al Virulence. 2018 Dec 31;9(1):648-658. doi
    Insert
    ttTi4348_ SEC _pvit-6_OspF_3'unc54
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    106366-plasmid-map-sequence-id-204924

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  13. BC-A1-016

    Plasmid
    #78704
    Purpose
    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78704-plasmid-map-sequence-id-171061

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  14. BC-A1-015

    Plasmid
    #78703
    Purpose
    A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78703-plasmid-map-sequence-id-171060

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  15. BC-A1-010

    Plasmid
    #78697
    Purpose
    A strong promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23100-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78697-plasmid-map-sequence-id-171055

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  16. BC-A1-008

    Plasmid
    #78695
    Purpose
    A weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78695-plasmid-map-sequence-id-171053

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  17. BC-A1-007

    Plasmid
    #78694
    Purpose
    A weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.
    Depositor
    Brian Chow
    Article
    Magaraci et al ACS Synth Biol. 2016 Apr 25.
    Insert
    BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    78694-plasmid-map-sequence-id-171052

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  19. pBP-J23108

    Plasmid
    #72964
    Purpose
    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    J23108 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72964-plasmid-map-sequence-id-447485

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  20. pBP-J23114

    Plasmid
    #72965
    Purpose
    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    J23114 promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72965-plasmid-map-sequence-id-447486

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  21. pBP-T7_RBS

    Plasmid
    #72978
    Purpose
    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72978-plasmid-map-sequence-id-447502

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  22. pTU2-B-RFP

    Plasmid
    #72955
    Purpose
    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 2 - B site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72955-plasmid-map-sequence-id-447542

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  23. pTU2-b-RFP

    Plasmid
    #72959
    Purpose
    Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 2 - b site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  25. Xlim-3 (lhx3)

    Plasmid
    #74139
    Purpose
    Partial cDNA clone for Xlim-3 (lhx3), named LH23-1. Cloned in BS KS+. To prepare RNA, sense: HindIII/T7; antisense: BamHI/T3.
    Depositor
    Igor Dawid
    Article
    Taira et al Dev Biol. 1993 Sep;159(1):245-56.
    Insert
    lhx3 (lhx3 Frog)
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  31. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid
    #70651
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. lentiCRISPR - C16orf80 sgRNA 2

    Plasmid
    #70650
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. lentiCRISPR - C3orf17 sgRNA 2

    Plasmid
    #70653
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C3orf17
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pNB682

    Plasmid
    #60143
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Use
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    A4V
    Promoter
    MET17pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60143-plasmid-map-sequence-id-96214

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Showing: 9501 - 9550 of 159258 results