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We narrowed to 24,955 results for: lars

Showing: 3961 - 3980 of 24955 results

  2. Epac-S-H189

    Plasmid
    #170350
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170350-plasmid-map-sequence-id-336138

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  3. pCAGGS-FuG-E (P440E)

    Plasmid
    #119978
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al J Neurosci Methods. 2018 Oct 19;311:147-
    Insert
    Fusion Glycoprotein type E (P440E)
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119978-plasmid-map-sequence-id-232261

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  4. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

    Plasmid
    #112060
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV2
    Use
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CMV
    Available since
    July 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    112060-plasmid-map-sequence-id-216934

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  6. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid
    #160567
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160567-plasmid-map-sequence-id-319681

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  7. pTT3-ecdICAM1-ST3-His

    Plasmid
    #210666
    Purpose
    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Synthetic, Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210666-plasmid-map-sequence-id-417657

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  8. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Use
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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  9. pCAGGS-FuG-C

    Plasmid
    #67512
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type C envelope coating virus particle with NeuRet.
    Depositor
    Kazuto Kobayashi
    Article
    Kato et al Hum Gene Ther. 2011 Dec;22(12):1511-23.
    Insert
    Fusion Glycoprotein type C
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67512-plasmid-map-sequence-id-116094

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  10. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

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  11. pTT3-ecdCD86-ST3-His

    Plasmid
    #210664
    Purpose
    Expression of the extracellular domain of human CD86 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human CD86 fused to Spytag003 and Histag (CD86 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    210664-plasmid-map-sequence-id-417658

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  13. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  14. pDGB3 omega1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2235)

    Plasmid
    #160646
    Purpose
    Module for the constitutive expression of the nptII, Cas9 and DsRed genes.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos
    Use
    CRISPR
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos, 35S, 35S
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    160646-plasmid-map-sequence-id-321668

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  15. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

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  16. pTT3-ecdCD80-ST3-His

    Plasmid
    #210665
    Purpose
    Expression of the extracellular domain of human CD80 fused to Spytag003 + Histag in HEK293 (or similar)
    Depositor
    Omer Dushek
    Article
    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.
    Insert
    Extracellular domain of human CD80 fused to Spytag003 and Histag (CD80 Human)
    Use
    Tags
    Histag and Spytag003
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210665-plasmid-map-sequence-id-443131

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  17. Epac-S-H171

    Plasmid
    #170353
    Purpose
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mTurq2Del_EPACdDEPCD_Q270E_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170353-plasmid-map-sequence-id-345820

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  18. pPSU2

    Plasmid
    #89566
    Purpose
    High copy number plasmid 2 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    750 bp EcoRV fragment
    3000 bp EcoRV fragment
    4000 bp EcoRV fragment
    50 bp PstI fragment
    100 bp PstI fragment
    200 bp PstI fragment
    300 bp PstI fragment
    400 bp PstI fragment
    500 bp PstI fragment
    600 bp PstI fragment
    1500 bp Psti fragment
    4100 bp PstI fragment
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89566-plasmid-map-sequence-id-177206

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  19. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

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  20. xPERT-hA2q

    Plasmid
    #231063
    Purpose
    Circularly permuted CasRx platform fused to human deaminase domain for A to I editing
    Depositor
    Meng How Tan
    Article
    Wang et al Nat Biotechnol. 2024 Oct 9. doi: 10.1038
    Inserts
    dCas13d
    Human deaminase ADAR2dd (ADARB1 Ruminococcus flavefaciens XPD3002)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    H460D, E488Q, only the deaminase domain (aa 276-7…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231063-plasmid-map-sequence-id-459741

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Showing: 3961 - 3980 of 24955 results