We narrowed to 93 results for: Free Use GFP
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Plasmid#218291PurposePlasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.DepositorInsertpURA3>KlURA3>tKlURA3-tPGK1
KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR3-deGFP-6xHis-T500
Plasmid#92224PurposeExpression of deGFP using the p70a promoter and UTR3DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70b-UTR1-deGFP-6xHis-T500
Plasmid#92222PurposeExpression of deGFP using the p70b promoter (weak version of the p70a promoter)DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70b (p70a promoter with one mutation)Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2_(-3kb)nrg1_typeII:GFP-CAAX
Plasmid#199394PurposepDEL63; transgenic construct to express GFP-CAAX under nrg1 type II regulatory region controlDepositorInserts(-3kb)nrg1 type II
GFP-CAAX
afp_3'_UTR
UseTol2 gateway destination vectorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500
Plasmid#92225PurposeExpression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidue Y35 mutated into TAG (Amber) stop codon f…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500
Plasmid#92227PurposeExpression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1DepositorInsertdeGFP Y35TAG D193TAG
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialMutationResidues Y35 D193 were mutated into TAG (Amber) s…Promoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBL7049
Plasmid#167219Purposecell-free arsenic sensing reporterDepositorInsertpArs-sfGFP
UseSynthetic BiologyAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7050
Plasmid#167220Purposecell-free mercury sensing reporterDepositorInsertpMer-sfGFP
UseSynthetic BiologyAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7051
Plasmid#167221Purposecell-free cadmium sensing reporterDepositorInsertpCad-sfGFP
UseSynthetic BiologyAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7053
Plasmid#167222Purposecell-free lead sensing reporterDepositorInsertpPbr-sfGFP
UseSynthetic BiologyAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7054
Plasmid#167223Purposecell-free copper sensing reporterDepositorInsertpCue-sfGFP
UseSynthetic BiologyAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7048
Plasmid#167218Purposecell-free nitrate sensing reporterDepositorInsertpNar-sfGFP
UseSynthetic BiologyMutationydfJ115 promoterAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUW mCherry-GFP-LC3
Plasmid#110060PurposeTo visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)DepositorInsertmCherry-GFP-LC3
UseLentiviralAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-N-Ezrin
Plasmid#202684Purposefluorescent protein tagged ezrinDepositorInsertMus musculus EZR(1-586):EGFP (Ezr Mouse)
ExpressionMammalianAvailable SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-C3-beta-spectrin
Plasmid#202685Purposefluorescent protein tagged beta-spectrinDepositorInsertEGFP:SPTB(1-2364) (SPTB Human)
ExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWF073_LVt-EF1a-eGFP-ffLuc-PGK-BlastR
Plasmid#233447PurposeStable expression of eGFP-firefly luciferase (ffLuc)DepositorInserteGFP-firefly luciferase (ffLuc)
UseLentiviralAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP-C1-FKBP-CYB5tail
Plasmid#202686Purposerapamycin-induced recruitment of proteins to ERDepositorInsertEGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
ExpressionMammalianAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-MmCENP-O-GFP
Plasmid#205178PurposeExpresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Mouse)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type mouse CENP-OPromoterT7Available SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-80-GFP
Plasmid#205182PurposeExpresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O N terminal …PromoterT7Available SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-298-5NS-GFP
Plasmid#205742PurposeExpresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O C-terminal …PromoterT7Available SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only