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We narrowed to 78 results for: FreeGenes

Showing: 21 - 40 of 78 results
  1. pOpen-T4gene30

    Plasmid
    #165554
    Purpose
    Ligase derived from T4 bacteriophage expressed in the early stage of lytic development. Has been implicated in T4 DNA synthesis and genetic recombination. May also play a role in T4 DNA repair.
    Insert
    T4-DNA Ligase
    Use
    Synthetic Biology
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pOpen-RNAse H

    Plasmid
    #165575
    Purpose
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.
    Insert
    RNAse H
    Use
    Synthetic Biology
    Available since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pOpen-TthRT

    Plasmid
    #165566
    Purpose
    DNA polymerase that efficiently reverse-transcribes RNA in the presence of MnCl2
    Insert
    Thermus thermophilus (Tth) RT
    Use
    Synthetic Biology
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pOpen-THEAQlig

    Plasmid
    #165535
    Purpose
    Thermostable ligase for incorporation of phosphorylated oligonucleotides during PCR and Ligase Chain Reaction
    Insert
    Taq DNA Ligase
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pOpen-THEAQpolA

    Plasmid
    #165512
    Purpose
    DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.
    Insert
    Taq DNA Polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Insert
    Eco31I
    Use
    Synthetic Biology
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. pOpen-UDG

    Plasmid
    #165531
    Purpose
    Uracil DNA glycosylase catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil.
    Insert
    UDG
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. pOpen-9N7lig

    Plasmid
    #165514
    Purpose
    9 Degrees North DNA Ligase active at elevated temperatures (45 - 90 degrees C).
    Insert
    9 Degrees North DNA Ligase
    Use
    Synthetic Biology
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Available since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pOpen-T7EI

    Plasmid
    #165540
    Purpose
    DNA Endonuclease; catalyzes cleavage of DNA mismatches and non-beta DNA structures (Holliday junctions and cruciform).
    Insert
    T7EI (T7 Endonuclease I)
    Use
    Synthetic Biology
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pOpen-T7gene5

    Plasmid
    #165534
    Purpose
    DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.
    Insert
    T7 DNA Polymerase (unmodified)
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  13. pOpen-K12polLF

    Plasmid
    #165511
    Purpose
    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.
    Insert
    DNA Polymerase I, Large (Klenow) Fragment
    Use
    Synthetic Biology
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  15. pOpen-Pwopol

    Plasmid
    #165562
    Purpose
    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.
    Insert
    Pwo DNA Polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  16. pOpen-T5gene122

    Plasmid
    #165544
    Purpose
    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.
    Insert
    T5 DNA Polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  17. pOpen-TthpolA

    Plasmid
    #165533
    Purpose
    DNA Polymerase whose activity is resistant to prolonged incubations at high temperatures (95 degrees C).
    Insert
    Tth DNA polymerase
    Use
    Synthetic Biology
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  18. pOpen-XbaIR

    Plasmid
    #165567
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.
    Insert
    XbaI
    Use
    Synthetic Biology
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  19. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 21 - 40 of 78 results