We narrowed to 59 results for: Westmeyer

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pU6_HEK3+1T>A_(PP7-C4-Q1)

Plasmid

#232437

Purpose

pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

Plasmid

#206956

Purpose

AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

mScarlet-I, MmMT3, QtEnc

Use

AAV

Tags

FLAG

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG-TGA-rt20s-antiGFP

Plasmid

#206953

Purpose

Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc, antiGFP-intrabody

Expression

Mammalian

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_CreNLS_PP7

Plasmid

#232439

Purpose

expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cre–MYC-NLS

Expression

Mammalian

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6_rd6_pegRNA_(PP7-C4-Q1)

Plasmid

#232433

Purpose

pegRNA with optimized 3' modifications to correct the rd6 mutation

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(3G)-mCherry

Plasmid

#206945

Purpose

Mammalian expression of heterobifunctional SasG linkers (3G)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (3G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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2M-Mx-FLAG

Plasmid

#206942

Purpose

Mammalian Expression of 2M-Mx-FLAG EMcapsulins

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, MxEnc

Tags

FLAG

Expression

Mammalian

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(7G)-mCherry

Plasmid

#206949

Purpose

Mammalian expression of heterobifunctional SasG linkers (7G)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (7G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(6G)-mCherry

Plasmid

#206948

Purpose

Mammalian expression of heterobifunctional SasG linkers (6G)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (6G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(5G)-mCherry

Plasmid

#206947

Purpose

Mammalian expression of heterobifunctional SasG linkers (5G)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (5G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(4G)-mCherry

Plasmid

#206946

Purpose

Mammalian expression of heterobifunctional SasG linkers (4G)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (4G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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sfGFP-SasG(2G)-mCherry

Plasmid

#206944

Purpose

Mammalian expression of heterobifunctional SasG linkers (2G)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

sfGFP-SaSasG (2G)-mCherry

Tags

mCherry and sfGFP

Expression

Mammalian

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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1M-Qt-FLAG

Plasmid

#206938

Purpose

Mammalian Expression of 1M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Tags

FLAG

Expression

Mammalian

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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3M-Qt-FLAG

Plasmid

#206940

Purpose

Mammalian Expression of 3M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, SeSmtA, TaEC1, QtEnc

Tags

FLAG

Expression

Mammalian

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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2M-Qt-FLAG

Plasmid

#206939

Purpose

Mammalian Expression of 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

MmMT3, QtEnc

Tags

FLAG

Expression

Mammalian

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pU6_HEK3_nsgRNA(PP7)

Plasmid

#232444

Purpose

nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

Depositor

Gil Westmeyer

Publication

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter

Use

CRISPR

Promoter

U6

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TGA-rt9us-antiGFP

Plasmid

#206954

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Expression

Mammalian

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Qt-FLAG-TAA-rt20s-antiGFP

Plasmid

#206955

Purpose

Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

Depositor

Gil Westmeyer

Publication

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

QtEnc, antiGFP-intrabody

Expression

Mammalian

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

Plasmid

#197265

Purpose

Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

Depositor

Gil Westmeyer

Publication

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPiggyBac_NTVE(PABP)

Plasmid

#254463

Purpose

Transposon encoding doxycycline-inducible NTVE(PABP). Co-expression of PiggyBac transposase required. Tet-On 3G and green fluorescent protein mGreenLantern are co-expressed via the CAG promoter.

Depositor

Gil Westmeyer

Publication

Armbrust et al Nat Commun. 2026 Apr 25;17(1):3812. doi:

Insert

NTVE(PABP)

Expression

Mammalian

Promoter

TRE3G

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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