We narrowed to 236 results for: tbp

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pMX-hTAF7

Plasmid

#44338

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF7 (TAF7 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Txnip

Plasmid

#178437

Purpose

doxycycline-inducible expression of Mouse Txnip in mammalian cells

Depositor

Filipe Pereira

Publication

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Txnip (Txnip Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-hTAF10

Plasmid

#44340

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF10 (TAF10 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R49A)

Plasmid

#112726

Purpose

Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R49A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R49A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF12

Plasmid

#44342

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF12 (Taf12 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(P51A)

Plasmid

#112728

Purpose

Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(P51A)

Tags

6His-TEV

Expression

Bacterial

Mutation

P51A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q54A)

Plasmid

#112730

Purpose

Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

Depositor

Joseph Wedekind

Publication

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q54A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q54A

Promoter

T7

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Plasmid

#132543

Purpose

Expresses CIRTS-1 (ORF5-TBP6.7-Pin domain-NLS) in mammalian cells

Depositor

Bryan Dickinson

Publication

Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

Insert

CIRTS-1: ORF5-TBP6.7-Pin domain-NLS

Tags

FLAG tag

Expression

Mammalian

Promoter

cmv d0

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR

Plasmid

#154001

Purpose

Vector expressed CasRx and sgRNA_Ptbp1 for AAV package

Depositor

Hui Yang

Publication

Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

Insert

CasRx, Ptbp1 sgRNAs

Use

AAV, CRISPR, and Mouse Targeting

Tags

FLAG

Promoter

GFAP, U6

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pMX-mTAF13

Plasmid

#44343

Depositor

Marc Timmers

Publication

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF13 (Taf13 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pRS-45

Plasmid

#40586

Depositor

Harald Schwalbe

Publication

Silvers et al Protein Expr Purif. 2012 Jul 24;85(1):14

Insert

yTAND12-TEV-hTBPc (TAF1, TBP Human, Budding Yeast)

Tags

His-tag and yTAND12

Expression

Bacterial

Promoter

T7

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Txnip(C247S)

Plasmid

#206353

Purpose

AAV plasmid expressing mutant TXNIP

Depositor

Connie Cepko

Publication

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C247S

Promoter

CMV

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-N.Txnip

Plasmid

#206348

Purpose

AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Publication

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

N terminal half (1-228aa)

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sC.Txnip

Plasmid

#206351

Purpose

AAV plasmid expressing deltion TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Publication

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

Short C terminal half (255-397aa)

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-C.Txnip(C247S)

Plasmid

#206349

Purpose

AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Publication

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C terminal half (149-397aa), C247S

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-nt.Txnip(C247S)(1-320aa)

Plasmid

#206352

Purpose

AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Publication

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

(1-320aa), C247S

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-C.Txnip(C247S_LL351-352AA)

Plasmid

#206350

Purpose

AAV plasmid expressing deltion and mutant TXNIP in retinal pigment epithelium

Depositor

Connie Cepko

Publication

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C terminal half (149-397aa), C247S and LL351&…

Promoter

human Best1

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-Txnip(C247S)

Plasmid

#206341

Purpose

AAV plasmid expressing mutant TXNIP in photoreceptors

Depositor

Connie Cepko

Publication

Xue et al Elife. 2021 Apr 13;10. pii: 66240. doi:

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C247S

Promoter

human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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