We narrowed to 9,400 results for: Coli

1. pET28b-MelJ

   Plasmid

   #225731

   Purpose

   To express MelJ (Rv1940) in E. coli

   Depositor

   Nicole Sampson

   Article

   Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

   Insert

   melJ
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB75

   Plasmid

   #226320

   Purpose

   E. coli protein expression for protein purification

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   6xHis-(Y to A MR1-6)-strep
   

   Use

   Tags

   6xHis and Strep

   Expression

   Bacterial

   Mutation

   N-terminal domain deleted, C-terminal domain dele…

   Promoter

   T7

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJB67

   Plasmid

   #226316

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with K to A CsoS2 MR1-6
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K261A, K320A, K380A, K436A, K495A, K546A

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJB74

   Plasmid

   #226314

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A, Y475A, Y534A

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJB73

   Plasmid

   #226313

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A, Y359A, Y420A

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJB72

   Plasmid

   #226312

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with Y to A CsoS2 MR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y300A

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJB45

   Plasmid

   #226304

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with C to S CsoS2 MR1-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C292S, C310S, C351S, C369S

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJB44

   Plasmid

   #226303

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   CsoS2 with C to S MR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C292S, C310S

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJB43

   Plasmid

   #226302

   Purpose

   E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

   Depositor

   David Savage

   Article

   Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

   Insert

   WT CsoS2, frameshift site intact
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTRC

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-a(+)-6xHis-miRFP(-5)-HO1

    Plasmid

    #219424

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Inserts

    miRFP682
    HO1
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Contains Ribosomal Binding Site for expression of…

    Promoter

    T7

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJBM2

    Plasmid

    #225289

    Purpose

    E. coli-Campylobacter shuttle vector; KanR

    Depositor

    Robert Moore

    Article

    McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

    Insert

    Replication elements from pCC311 for pJBM2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSF-coSnaA-TEV-His_His-TEV-SnaC

    Plasmid

    #225059

    Purpose

    recombinant expression of SnaA in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

    Insert

    SnaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-ChmN-C47A

    Plasmid

    #221367

    Purpose

    Express C47A ChmN in E. coli

    Depositor

    Emily Balskus

    Article

    Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

    Insert

    chmN-C47A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed Cys 47 to Ala

    Promoter

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid

    #208628

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 1~41, other residues not included

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid

    #208625

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 72~197, other residues not included

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-6xHis-GFP(+6)-L1

    Plasmid

    #205039

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(+6)-L1
    

    Use

    Tags

    GRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-6xHis-GFP(+6)-L2

    Plasmid

    #205040

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(+6)-L2
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-6xHis-GFP(+6)-L3

    Plasmid

    #205041

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(+6)-L3
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-6xHis-GFP(+6)-H1

    Plasmid

    #205042

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(+6)-H1
    

    Use

    Tags

    KRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-6xHis-GFP(+6)-H2

    Plasmid

    #205043

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(+6)-H2
    

    Use

    Tags

    KRRRKKKRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-6xHis-GFP(+6)-H3

    Plasmid

    #205044

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(+6)-H3
    

    Use

    Tags

    KRRRKKKRRRKKKRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET-6xHis-GFP(-6)-H2_mCherry(-6)

    Plasmid

    #205049

    Purpose

    Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(-6)-H2_mCherry(-6)
    

    Use

    Tags

    MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-6xHis-GFP(0)-L1

    Plasmid

    #205033

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-L1
    

    Use

    Tags

    GRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-6xHis-GFP(0)-L2

    Plasmid

    #205034

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-L2
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-6xHis-GFP(0)-L3

    Plasmid

    #205035

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-L3
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET-6xHis-GFP(0)-H1

    Plasmid

    #205036

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-H1
    

    Use

    Tags

    KRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-6xHis-GFP(0)-H2

    Plasmid

    #205037

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-H2
    

    Use

    Tags

    KRRRKKKRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-6xHis-GFP(0)-H3

    Plasmid

    #205038

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-H3
    

    Use

    Tags

    KRRRKKKRRRKKKRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid

    #208626

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 191~307, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge