We narrowed to 13,078 results for: SHI

1. pCAG-All-in-one-hCascade

   Plasmid

   #134919

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHes7-Achilles-Hes7

   Plasmid

   #153528

   Purpose

   Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

   Depositor

   Ryoichiro Kageyama

   Article

   Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

   Insert

   Hes7 (Hes7 Mouse)
   

   Use

   Tags

   Achilles

   Expression

   Mammalian

   Mutation

   Promoter

   mouse Hes7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pP{ELAV-GeneSwitch}

   Plasmid

   #83957

   Purpose

   Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

   Depositor

   Haig Keshishian

   Article

   Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

   Insert

   fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Human, Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   elav

   Available since

   Dec. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP-GFP-CERT(PHD)

   Plasmid

   #221746

   Purpose

   Stable expression of GFP-CERT(PHD)in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   CERT (CERT1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–116

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IP-EGFP-LOV2-Cyb5a

   Plasmid

   #233348

   Purpose

   Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Cyb5a (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted (Cyb5a 95-134) and N538E

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

   Plasmid

   #172104

   Purpose

   Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. HR180-LGR5-iCT

   Plasmid

   #129094

   Purpose

   KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

   Depositor

   Toshiro Sato

   Article

   Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

   Insert

   iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
   

   Use

   Homologous recombination donor plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS0045

   Plasmid

   #131124

   Purpose

   Mammalian Target-ACE expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1, TadA, and UGI

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

   Promoter

   CMV promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-hPPP3CA-FLAG

   Plasmid

   #179134

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX209-neo-HA-Ub-T2A-Tet3G

    Plasmid

    #202436

    Purpose

    Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Ubiquitin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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