We narrowed to 20,245 results for: ACE;

1. pET21b-Caspase-3(C163S)

   Plasmid

   #90088

   Purpose

   Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   C163S

   Promoter

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Halo-Tev-Flag-Ube2v2

   Plasmid

   #110070

   Purpose

   Express Halo-Tev-Flag-Ube2v2 (human) in mammalian cells

   Depositor

   Yimon Aye

   Article

   Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

   Insert

   Halo-Tev-Flag-Ube2v2 (UBE2V2 Human)
   

   Use

   Tags

   Halo-TEV-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. nuc-GafD-mTurboID-HA

   Plasmid

   #184641

   Purpose

   Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   nuc-GlycoID-HA
   

   Use

   Tags

   3xHA tag and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   GafD -> expressed 22-178 ; BirA* -> express…

   Promoter

   CMV

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cox7c-pHLuorin-sems

   Plasmid

   #107766

   Purpose

   labeling of mitochondrial cytochrome c oxidase, pH measurements

   Depositor

   Karin Busch

   Article

   Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

   Insert

   Subunit Cox7c fused to pHluorin (COX7C Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)

   Plasmid

   #156463

   Purpose

   Express Heme Oxygenase (Nostocaceae) and ReBphP-PCM

   Depositor

   Andre Stiel

   Article

   Mishra et al Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi

   Inserts

   Heme Oxygenase
   ReBphP-PCM
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBAD-iLACCO1

   Plasmid

   #218817

   Purpose

   Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBAD-iLACCO1.1

   Plasmid

   #218818

   Purpose

   Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD-iLACCO1.2

   Plasmid

   #218819

   Purpose

   Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBAD-DiLACCO1

   Plasmid

   #218820

   Purpose

   Bacterial expression of genetically encoded green fluorescent L-lactate control biosensor

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   DiLACCO1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAH-CTX1-rhadCas9

    Plasmid

    #129391

    Purpose

    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Burkholderia cenocepacia codon-optimized dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Entire dCas9 codon optimized for the GC-rich B. c…

    Promoter

    PrhaBAD

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET26b_Nb.AD101

    Plasmid

    #131402

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD101
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GW1-rogRFP2

    Plasmid

    #207876

    Purpose

    Mammalian expression of the red fluorescent rogRFP2 redox sensor

    Depositor

    Mathew Tantama

    Article

    Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

    Insert

    rogRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAV125

    Plasmid

    #63191

    Purpose

    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBTK301

    Plasmid

    #110593

    Purpose

    BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Bba_B0015 Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pBTK552

    Plasmid

    #110618

    Purpose

    BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI.

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pcDNA3-Lyn-dPlcR(K30/32L)

    Plasmid

    #181833

    Purpose

    Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-dPlcR (K30L,K32L)
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, dd…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK4_002

    Plasmid

    #123811

    Purpose

    Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SV40 pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127321

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBTK519

    Plasmid

    #110603

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK2_007

    Plasmid

    #123702

    Purpose

    Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pEF1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDHblast-flx-sORF-Katushka

    Plasmid

    #179457

    Purpose

    Lentiviral vector encoding a CRE inducible Katushka gene

    Depositor

    Ton Schumacher

    Article

    Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

    Insert

    loxP-shuffledORF-loxP-Katushka
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Cav2.1 HA pCAGGS

    Plasmid

    #206076

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCPP5233

    Plasmid

    #128715

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

    Insert

    shcM-hopM1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Halo-Tev-Flag-Ube2v1

    Plasmid

    #110069

    Purpose

    Express Halo-Tev-Flag-Ube2v1 (human) in mammalian cells

    Depositor

    Yimon Aye

    Article

    Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

    Insert

    Halo-Tev-Flag-Ube2v1 (UBE2V1 Human)
    

    Use

    Tags

    Halo-TEV-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-Akt-Scramble

    Plasmid

    #175014

    Purpose

    Negative-control construct for Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-Scramble
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-Akt-STOPS-NES

    Plasmid

    #175008

    Purpose

    Cytosol-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabe-FLAG-Neuroserpin

    Plasmid

    #58261

    Purpose

    Retroviral vector for expressing Neuroserpin in mammalian cells

    Depositor

    Joan Massague

    Article

    Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

    Insert

    Neuroserpin (SERPINI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUT_mNF-Cas13d-Triplex

    Plasmid

    #227709

    Purpose

    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Insert

    mCherry-P2A-RfxCas13d-Triplex
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available Since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Teto-FUW-Pax6

    Plasmid

    #61541

    Purpose

    Expresses Pax6 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Pax6 (Pax6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only