We narrowed to 4,679 results for: cre encoding plasmid

1. FLAG-JAK1Gly590Arg

   Plasmid

   #174573

   Purpose

   Human FLAG-tagged JAK1 Gly590Arg mutant expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   JAK1Gly590Arg
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Gly590Arg

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

   Plasmid

   #163138

   Purpose

   Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   PLK1 (PLK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pECE-M2-BAIAP2 S366A

   Plasmid

   #31657

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   BAI1-associated protein 2 S366A (BAIAP2 Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   changed Serine 366 to Alanine

   Promoter

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

4. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-αIFNα-ib

   Plasmid

   #110798

   Purpose

   Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells

   Depositor

   Thomas Böldicke

   Article

   Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

   Insert

   intrabody against mouse interferon alpha
   

   Use

   Tags

   ER retention signal (SEKDEL), ER signal peptide, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcBac3-EcTrp-TGA-EGFP**

   Plasmid

   #228786

   Purpose

   EcTrp aaRS/tRNA system for TGA suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcTrpRS
   EcWtR
   EGFP**
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TGA suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR - EGFP sgRNA 2

   Plasmid

   #51761

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPR - EGFP sgRNA 4

   Plasmid

   #51763

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCRISPR - EGFP sgRNA 5

   Plasmid

   #51764

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 5
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pECE-M2-AMPKalpha2 M93G

    Plasmid

    #31653

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Methionine 93 to Glycine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pECE-HA-AMPKalpha2 M93G

    Plasmid

    #31655

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    changed Methionine 93 to Glycine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

    Plasmid

    #105023

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

    Plasmid

    #105024

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-U6-MbCas12a crRNA-BsmBI cassette (BPK4449)

    Plasmid

    #114088

    Purpose

    U6 promoter crRNA entry vector used for all MbCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for MbCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-shCasp2-IRES-GFP

    Plasmid

    #52061

    Purpose

    stable knockdown of Caspase-2 in mammalian cells

    Depositor

    Trudy Oliver, Tyler Jacks

    Article

    Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

    Insert

    Caspase-2 shRNA (CASP2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pET-pro-siRNA-V2-LMNA

    Plasmid

    #111088

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pECE-M2-CDC27 S379A

    Plasmid

    #31662

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog S379A (CDC27 Human)
    

    Use

    Tags

    M2

    Expression

    Mammalian

    Mutation

    changed Serine 379 to Alanine

    Promoter

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only