We narrowed to 6,501 results for: nls

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pColADuet-DddA-DddI-WT

Plasmid

#254736

Purpose

Expresses Simiaoa sunii DddA(SsDddA) and DddI(SsDddI) in BL21(DE3) cells

Depositor

Andrew Stergachis

Publication

Swanson et al Nat Biotechnol. 2025 Dec 3. doi: 10.1038

Inserts

DddA
DddI

Tags

N-6His tag

Expression

Bacterial

Available since

Aug. 3, 2026

Availability

Academic Institutions and Nonprofits only

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PColDuet-nHis-SsDddI.dna

Plasmid

#254901

Purpose

Expresses of Simiaoa sunii DddI(SsDddI) in BL21(DE3) cells

Depositor

Andrew Stergachis

Publication

Swanson et al Nat Biotechnol. 2025 Dec 3. doi: 10.1038

Insert

SsDddI

Tags

6xHis

Expression

Bacterial

Promoter

T7 promoter

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaCIDR

Plasmid

#157872

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDR

Depositor

Nicolas Fawzi

Publication

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaCIDR

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_D290V

Plasmid

#139110

Purpose

Express FL hnRNPA2 D290V with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Publication

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

D290V

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 S12xQ

Plasmid

#127193

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 S12xQ (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR1075

Plasmid

#111093

Purpose

Expresses eGFP donor in pUC19

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eGFP

Expression

Mammalian

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-N-PrLD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCR1063

Plasmid

#111091

Purpose

Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

Depositor

Jacob Corn

Publication

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

dCas9-KRAB

Use

Lentiviral

Tags

mCherry

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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HA-MDFI

Plasmid

#207836

Purpose

Tagged MDFI for western blotting and imaging

Depositor

Charles Cox

Publication

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MDFI (MDFI Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_Q331K

Plasmid

#98673

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

Depositor

Nicolas Fawzi

Publication

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

Q331K

Available since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-RARalpha (T181A,S445A,S461A)

Plasmid

#35556

Depositor

Jonathan Kurie, Harish Srinivas

Publication

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

Flag and His

Expression

Mammalian

Mutation

T181A, S445A, S461A

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pPLS24

Plasmid

#17285

Depositor

Nancy Craig

Publication

Sharpe et al EMBO J. 1998 Oct 1. 17(19):5822-31.

Insert

TnsD (tnsD E. coli transposon Tn7)

Tags

His-tag

Expression

Bacterial

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-AR C619Y

Plasmid

#86429

Purpose

Fluorescent human androgen receptor (fused to EGFP) with point mutation C619Y

Depositor

Michael Mancini

Publication

Nazareth et al Mol Endocrinol. 1999 Dec;13(12):2065-75.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Androgen receptor (AR) (AR Human)

Tags

EGFP

Expression

Mammalian

Mutation

C619Y

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pTHMT_hnRNPF_FL

Plasmid

#139114

Purpose

express FL hnRNPF with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

None

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDIV489

Plasmid

#177697

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hnRNPA2_190-303

Plasmid

#118822

Purpose

Bacterial expression of hnRNPA2 truncation (residues 190 to 302).

Depositor

Nicolas Fawzi

Publication

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His tag and TEV

Expression

Bacterial

Mutation

contains amino acids 190-302

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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