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pEGFP-C1-AR C619Y
(Plasmid #86429)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 86429 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech (Takara)
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 7936
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Androgen receptor (AR)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2760
  • Mutation
    C619Y
  • GenBank ID
    NM_000044.4
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-AR C619Y was a gift from Michael Mancini (Addgene plasmid # 86429 ; http://n2t.net/addgene:86429 ; RRID:Addgene_86429)
  • For your References section:

    A C619Y mutation in the human androgen receptor causes inactivation and mislocalization of the receptor with concomitant sequestration of SRC-1 (steroid receptor coactivator 1). Nazareth LV, Stenoien DL, Bingman WE 3rd, James AJ, Wu C, Zhang Y, Edwards DP, Mancini M, Marcelli M, Lamb DJ, Weigel NL. Mol Endocrinol. 1999 Dec;13(12):2065-75. 10.1210/mend.13.12.0382 PubMed 10598582