We narrowed to 20,388 results for: ACE;

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pcDNA3-Akt-Scramble

Plasmid

#175014

Purpose

Negative-control construct for Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-Scramble

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBbB8k-csg-nb112

Plasmid

#171702

Purpose

Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.

Depositor

Neel Joshi

Article

Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

Insert

csgBACEFG, with nanobody fused to CsgA

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Akt-STOPS-NES

Plasmid

#175008

Purpose

Cytosol-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

mCherry-Akt-STOPS-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pCAGGS

Plasmid

#206076

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCPP5233

Plasmid

#128715

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

shcM-hopM1

Expression

Bacterial

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_007

Plasmid

#123702

Purpose

Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pEF1a

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMT2 Beta1b GFP

Plasmid

#89893

Purpose

Mammalian expression of Beta1b GFP

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

Insert

Cacnb1 (Cacnb1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

GFP sequence inserted just before the Stop codon

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK301

Plasmid

#110593

Purpose

BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Bba_B0015 Terminator

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMT2 Beta1b mCherry

Plasmid

#89892

Purpose

Mammalian expression of Beta1b mCherry

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

Insert

Cacnb1 (Cacnb1 Rat)

Tags

mCherry

Expression

Mammalian

Mutation

mCherry sequence inserted just before the Stop co…

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Worm and Yeast

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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MTK4_003

Plasmid

#123812

Purpose

Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

rglb pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-optimizedNedd4.2d

Plasmid

#165103

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

Insert

Nedd4.2d (NEDD4L Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Mutation

E66T

Promoter

CAG

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK402

Plasmid

#110598

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe-FLAG-Neuroserpin

Plasmid

#58261

Purpose

Retroviral vector for expressing Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
BE3-Gam

Plasmid

#100807

Purpose

Expresses BE3-Gam in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

BE3-Gam

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pUT_mNF-Cas13d-Triplex

Plasmid

#227709

Purpose

LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

mCherry-P2A-RfxCas13d-Triplex

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV-d2i

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pSB3K3 containing mRFP1

Plasmid

#118083

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLX307 hcRed1

Plasmid

#117732

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1K3 containing mRFP1

Plasmid

#118082

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AktAR2-NES

Plasmid

#175011

Purpose

Cytosol-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK569

Plasmid

#110614

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Expression

Bacterial

Mutation

V32M (see comments below)

Available Since

Oct. 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK501

Plasmid

#110602

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-AktAR2-NLS

Plasmid

#175012

Purpose

Nuclear-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

AktAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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CDA1-BE3

Plasmid

#100804

Purpose

Expresses CDA1-BE3 in mammalian cells

Depositor

David Liu

Article

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

CDA1-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAcBac1-haloTyrRS C6

Plasmid

#141174

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Article

Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

Insert

C6 HaloTyr tRNA synthetase

Use

Lentiviral

Expression

Mammalian

Mutation

L270S, Y271L, N311G, C313T

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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