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Plasmid#191548PurposeProtein expression of HPV16 E7 E80A/D81ADepositorInsertHPV16
UseRetroviralTagsFlagHAExpressionMutationE80A/D81APromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-SpGalpha-i
Plasmid#185568Purposeto see protein dynamicsDepositorInsertGalpha-i
UseIn vitro transcription (mrna synthesis)TagsGFP, S-TagExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpPLK1-GFP
Plasmid#185573Purposeto see protein dynamicsDepositorInsertPLK1
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpPLK1L-GFP
Plasmid#185574Purposeto see protein dynamicsDepositorInsertPLK1L
UseIn vitro transcription (mrna synthesis)TagsGFPExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS Coupler 6
Plasmid#185829PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].DepositorInsert(GlyGlyGlyGlySer)x3 linker
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS Coupler 5
Plasmid#185828PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].DepositorInsert(GlyGlyGlyGlySer)x3 linker
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS Coupler 4
Plasmid#185827PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].DepositorInsert(GlyGlyGlyGlySer)x3 linker
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS Coupler 2
Plasmid#185825PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].DepositorInsert(GlyGlyGlyGlySer)x3 linker
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS Coupler 3
Plasmid#185826PurposeCloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].DepositorInsert(GlyGlyGlyGlySer)x3 linker
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNK132d
Plasmid#156370PurposeExpresses heterlogous ribosomal proteinsDepositorInsertS20, S16, S1, S15 ribosomal genes
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL1275
Plasmid#129386PurposeExpression plasmid with pLL1270 as backbone and alpha-Xylp_1211, alpha-L-Galp_697, alpha-L-Araf_1120 and beta-Xylp_1710 as insertsDepositorInsertsalpha-Xylp_1211 (Ga0256695_ 1211)
beta-Xylp_1710
alpha-L-AraF_1120
alpha-L-Galp_697
UseTagsExpressionBacterialMutationPromoterClostridium thermocellum DSM1313 Cellobiose Phosp…Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIAGO-AceF1
Plasmid#114461PurposeExpression of late polyene biosynthetic genes in streptomycetesDepositorInsertAceDI + AceDII + AceN + AceM
UseTagsExpressionBacterialMutationPromoterermEAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBsaGSPdR
Plasmid#108205PurposePdRDepositorInsertPutidaredoxin reductase
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHPro54G
Plasmid#108204Purpose4th Pro5DepositorInsertPro-Pro-Pro-Pro-Pro
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable sinceApril 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGreenII Tfs-qqr::LUC
Plasmid#44466PurposeExpresses a luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutationDepositorInsertA luciferase gene disrupted by a QQR ZFN target site and a frame-shift mutation
UseLuciferase; Plant transformationTagsExpressionMutationA disruption was made between the first and secon…Promoter35S PromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-ssRFP-GFP-KDEL
Plasmid#128257PurposeFluorescence reporter to monitor ER-phagy activityDepositorInsertssRFPGFPKDEL
UseLentiviralTagsRFPGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
HT041_FCMV_CheRiff_CFP
Plasmid#136636Purposeencodes CheRiff channelrhodopsin for lentiviral transductionDepositorInsertCheRiff-CFP
UseLentiviralTagsCFPExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGL815
Plasmid#52322PurposeA vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.DepositorTypeEmpty backboneUseAgrobacteriumTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-WGA-mCherry
Plasmid#224119PurposeTo express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.DepositorInsertWGA-mCherry
UseAAVTagsWGA and mCherryExpressionMammalianMutationAll mutations related to the WGA in this construc…PromoterCAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPING
Plasmid#26046DepositorInsertSS peptide-LacZ-KHHHHHH
UseTagsKHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eGFP_ER2
Plasmid#209282PurposeExpresses FR-GECO1c and CoChR in the zebrafish heartDepositorInsertFR-GECO1c_P2A_CoChR_KGC_eGFP_ER2
UseZebrafish expressionTagsExpressionMutationPromoterzACNE20_myl7Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2
Plasmid#113634PurposeAn expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepaciaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-FOXM1-shRNA-81
Plasmid#89495PurposeLentiviral vector with constitutive expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(empty)-TAG
Plasmid#138209PurposeTo facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' regionDepositorTypeEmpty backboneUseT7 transcriptionTagsTAG sequenceExpressionMammalianMutationPromoterCMV Promoter, T7 PromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSALECT-EcoBam
Plasmid#59705PurposeFolding reporter with ampicillin resistance as a readoutDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCONR221
Plasmid#216146PurposeGateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistanceDepositorTypeEmpty backboneUseCloning backboneTagsExpressionMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xmEGFP
Plasmid#127800PurposeHomo-trimer mEGFP-mEGFP-mEGFP expression vectorDepositorInsertmEGFP-mEGFP-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINE
Plasmid#26043DepositorInsertHis-lacZ-KHHHHHH
UseTagsKHHHHHH and MAHHHHHHSSGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPINM
Plasmid#26044DepositorInsertHis-MBP-3C-lacZ
UseTagsMAHHHHHHSSG_MBP_LEVLFQGPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (C-gap-WT)
Plasmid#105922PurposeAAV-mediated gene expression from CBh promoter. Contains left AAV2 ITR with an 11bp deletion in C region and canonical right AAV2 ITRDepositorInsertsmKate2
IRES-Multiple cloning site
UseAAVTagsExpressionMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBJ077_zACNE20_E1b_2xLyn_mCherry
Plasmid#209281PurposeExpresses membrane-labelled mCherry in the zebrafish heartDepositorInsertmCherry2
UseZebrafish expressionTagsExpressionMutationPromoterzACNE20_E1bAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINVL
Plasmid#26040DepositorInsertu-LacZ
UseTagsu-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPINVH
Plasmid#26041DepositorInsertu-LacZ-KHHHHHH
UseTagsKHHHHHH and u-phosphatase secretion leaderExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO-FOXM1-shRNA-84
Plasmid#89494PurposeLentiviral vector with constitutive expression of shRNA targeting human FOXM1.DepositorInsertFOXM1 (FOXM1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWP78
Plasmid#61263PurposeSmall IncP-based broad host range replicating vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-XBP1u-FLuc
Plasmid#31239DepositorInsertFLAG-XBP1u-FLuc (XBP1 Human)
UseTagsFlag and FlucExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 TNRC6C
Plasmid#20023DepositorInsertTrinucleotide repeat containing 6C (TNRC6C Human)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsPatL1_I
Plasmid#146547PurposeMammalian Expression of HsPatL1DepositorInsertHsPatL1 (PATL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only