We narrowed to 5,559 results for: SUP

1. IL2R/hE-cadherin-cytotail

   Plasmid

   #45773

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Interleukin-2 receptor α subunit extracellular an…

   Promoter

   CMV

   Available since

   June 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTagGFP2-N SIN1 deltaNCD

   Plasmid

   #124920

   Purpose

   For expression of N-terminal Conserved Domain deletion mutant of SIN1-GFP (delta2-137)

   Depositor

   Jin Chen

   Article

   Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

   Insert

   MAPKAP1 (MAPKAP1 Human)
   

   Use

   Tags

   TagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_iRFP_P2AT2A_mScarlet-I_Myl9

   Plasmid

   #172474

   Purpose

   Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

   Depositor

   Sean R Collins

   Article

   Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

   Inserts

   iRFP670
   mScarlet-I-Myl9 (MYL9 Human)
   

   Use

   Lentiviral

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha (with UCOE) and none (same transcript as…

   Available since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF019 MBP-β-catenin-His

   Plasmid

   #154070

   Purpose

   Expresses MBP-β-catenin-His (human β-catenin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK-VIM270-gSM

   Plasmid

   #215870

   Purpose

   This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

   Depositor

   Kumiko Ui-Tei

   Article

   Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

   Insert

   3 tandem repeats of target site (complementary to seed seq of guide strand of siRNA for VIM) is inserted to 3'UTR of RLuc (VIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-VIM270-gCM

   Plasmid

   #215868

   Purpose

   This plasmid encodes luciferaase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.

   Depositor

   Kumiko Ui-Tei

   Article

   Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d

   Insert

   target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJet-CMV-IARS1-GFP

   Plasmid

   #212322

   Purpose

   EGFP-tagged IARS1

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Puro-mGSDME F2A

   Plasmid

   #154878

   Purpose

   Expresses mGSDME F2A in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   changes Phenylalanine 2 to Alanine

   Promoter

   CMV

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV-AR-C619Y

   Plasmid

   #171222

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: C619Y, mutation at the end of zinc finger 2.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-C619Y (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-C619Y

   Promoter

   EF-1a

   Available since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

    Plasmid

    #176060

    Purpose

    Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K164A and H165A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUW-ubiquitin-EphB4-SV40-GFP (B4G)

    Plasmid

    #65444

    Purpose

    Expression of human EPHB4 and GFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    EPHB4 (EPHB4 Human)
    

    Use

    Lentiviral

    Tags

    SV40-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBQ

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

    Plasmid

    #176059

    Purpose

    Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgSmarca4#1/Cre

    Plasmid

    #173619

    Purpose

    Expresses a Smarca4-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Smarca4 (Smarca4 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-sgSmarca4#2/Cre

    Plasmid

    #173620

    Purpose

    Expresses a Smarca4-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Smarca4 (Smarca4 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMG059 MBP-CREB-His

    Plasmid

    #154076

    Purpose

    Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CREB1 (CREB1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CD40-bio-His

    Plasmid

    #51648

    Purpose

    Expresses full-length Tumor necrosis factor receptor superfamily member CD40 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    CD40 molecule (CD40 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV2-CAG-3xNLS-AausFP1

    Plasmid

    #191096

    Purpose

    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

    Depositor

    Pantelis Tsoulfas, Murray Blackmore

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    3xNLS-AausFP1
    

    Use

    AAV and Synthetic Biology

    Tags

    Three repeats of the nuclear localizzation seque…

    Expression

    Mammalian

    Mutation

    Optimized to Human codon usage

    Promoter

    CMV enhancer and CAG

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CMV-MRLC1-mRuby2-IRES-PuroR

    Plasmid

    #103031

    Purpose

    Lentiviral expression of mRuby2-tagged MRLC with puromycin selection

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

    Plasmid

    #204547

    Purpose

    Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

    Insert

    NBR1 (NBR1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deltaUBA

    Promoter

    Available since

    Aug. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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