We narrowed to 5,803 results for: SUP

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GFP Cav2.2 DomI + DomII pMT2

Plasmid

#206095

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tag

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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An HA CAAX pMT2

Plasmid

#206116

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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An CAAX pMT2

Plasmid

#206118

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 D122A pcDNA3

Plasmid

#206068

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K62D/K64D

Plasmid

#202786

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K62D/K64D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K62D, K64D

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGG-GST-TEV-NAP1 (WT)

Plasmid

#198034

Purpose

Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NAP1 (AZI2 Human)

Tags

GST-TEV

Expression

Mammalian

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

Plasmid

#198072

Purpose

Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV-EGFP

Expression

Insect

Mutation

TBK1ΔCTD (res 1-666)

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SOD1 C57D/C146D

Plasmid

#191848

Purpose

express C57D/C146D mutant SOD1 in mammalian cells

Depositor

Nam-Chul Ha

Article

Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

C57D, C146D

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pProEx-HTa-SOD1 C57A/C146A

Plasmid

#191850

Purpose

express C57A/C146A mutant SOD1 in bacterial cells

Depositor

Nam-Chul Ha

Article

Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

Insert

SOD1 (SOD1 Human)

Expression

Bacterial

Mutation

C57A, C146A

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pProEx-HTa-SOD1 C6A/C111A

Plasmid

#191852

Purpose

express C6A/C111A mutant SOD1 in bacterial cells

Depositor

Nam-Chul Ha

Article

Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

Insert

SOD1 (SOD1 Human)

Expression

Bacterial

Mutation

C6A, C111A

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 WWE-PARP

Plasmid

#192548

Purpose

pENTR plasmid Triticum aestivum sro1, amino acids 1-434, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)

Use

Gateway entry vector

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINF Ta-sro1 PARP

Plasmid

#192549

Purpose

E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)

Tags

His6, 3C protease cleavage site

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-1

Plasmid

#190095

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-1 (ABHD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

deleted the base pairs 6704-6709 refer to NM_0070…

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-2

Plasmid

#190096

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-2 (ABHD2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

deleted the base pairs 7122-7129 refer to NM_0070…

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGST2-GST-TEV-OPTN S177D S473D

Plasmid

#187827

Purpose

Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

OPTN (OPTN Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

S177D S473D

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRbm10#2/Cre

Plasmid

#173651

Purpose

Expresses a Rbm10-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rbm10 (Rbm10 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRnf43#2/Cre

Plasmid

#173614

Purpose

Expresses a Rnf43-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Rnf43 (Rnf43 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgUbr5#2/Cre

Plasmid

#173624

Purpose

Expresses a Ubr5-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ubr5 (Ubr5 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgWrn#1/Cre

Plasmid

#173625

Purpose

Expresses a Wrn-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Wrn (Wrn Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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