We narrowed to 6,946 results for: crispr cas9 plasmids

1. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgRNA-lib 2.0

   Plasmid

   #89638

   Purpose

   sgRNA expression in mammalian cells after Gateway cloning

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HCP4

   Plasmid

   #166106

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP6

   Plasmid

   #166108

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)

   Depositor

   Matthias Heinemann

   Insert

   Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP973-1

   Plasmid

   #99496

   Purpose

   TEV::linker::meGFP:3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::linker::meGFP::3XFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BB3nK_ext_AC

   Plasmid

   #108686

   Purpose

   Plasmid for assembly of donor DNA templates consisting of two expression units

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BB3nK_ext_AD

   Plasmid

   #108687

   Purpose

   Plasmid for assembly of donor DNA templates consisting of three expression units

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAS_sfGFP150K

   Plasmid

   #193313

   Purpose

   pAS control plasmid for sfGFP expression

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   super folder GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150K in sfGFP

   Promoter

   EF1

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWD509

    Plasmid

    #73739

    Purpose

    Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-FLP-D5
    

    Use

    Tags

    Expression

    Worm

    Mutation

    G5D

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS328

    Plasmid

    #73717

    Purpose

    C. elegans germline CRE expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    2xNLS-CRE
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAP974-1

    Plasmid

    #99497

    Purpose

    TEV::linker::tagRFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::linker::tagRFP::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAP997-1

    Plasmid

    #99500

    Purpose

    3XFlag::tagRFP::linker::TEV

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::tagRFP::linker::TEV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAP975-1

    Plasmid

    #99498

    Purpose

    TEV::linker::tagBFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::linker::tagBFP::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAP758-1

    Plasmid

    #99494

    Purpose

    TEV::eGFP::3XFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    TEV::eGFP::3XFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAP546-2

    Plasmid

    #99476

    Purpose

    3XFlag::mNeptune::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::mNeptune::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAP693-3

    Plasmid

    #99492

    Purpose

    nirFP::3xFlag

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    nirFP::3xFlag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAP998-5

    Plasmid

    #99501

    Purpose

    3XFlag::tagBFP::linker::TEV

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::tagBFP::linker::TEV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAP576-2

    Plasmid

    #99481

    Purpose

    3XFlag::tagBFP::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::tagBFP::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330_REC8_cterm

    Plasmid

    #222522

    Purpose

    Cas9/sgRNA plasmid for targeting REC8

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    Cas9, REC8 sgRNA (REC8 Synthetic, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge